Yulmira Yanti, Hasmiandy Hamid, Nurbailis Nurbailis, Yaherwandi Yaherwandi, Yenny Liswarni, Ilham Wibowo, Sisxri Selviana


Bacterial leaf blight, caused by Xanthomonas axonopodis pv. allii, poses a substantial risk to shallot crops. Actinobacteria are promising candidates for biological control because of their ability to generate bioactive compounds. This research aimed to identify the most effective Actinobacteria isolates for managing bacterial leaf blight and boosting the growth and yield of shallot plants. The study was conducted in three phases: 1.) Isolation and characterization of Actinobacteria and X. axonopodis pv. allii, 2.) Selection of Actinobacteria to control X. axonopodis pv. allii and boost shallot plant growth with 21 treatments and three replications, and 3.) Molecular identification and testing of selected Actinobacteria for controlling X. axonopodis pv. allii. This research was carried out in the microbiology laboratory and experimental farm of the Faculty of Agriculture at Andalas University in West Sumatra, Indonesia. The study identified several indigenous Actinobacteria isolates (AT2aP7Y, AS3aA7Y, AA2aP7Y, AS1aP6Y, and AS1aP7Y) with high phosphate solubilizing indices, strong antagonistic activity, and significant abilities in nitrogen fixation and phytohormone (IAA) production. These isolates were identified as Streptomyces spp. and Micromonospora shersina R-Ac134 through 16sRNA sequencing. They demonstrated potential for effectively controlling bacterial leaf blight and enhancing the growth and yield of shallot plants. In conclusion, the indigenous Actinobacteria isolates from the regions of Solok, Tanah Datar, and Agam in West Sumatra show promise as biological control agents against bacterial leaf blight in shallot plants, while also promoting plant growth and productivity.


Actinobacteria, shallots, exploration, Xanthomonas axonopodis pv. allii

Full Text:



Aeny, T. N., J. Prasetyo, R. Suharjo, S.R. Dirmawati, Efri, and A. Niswati. 2018. Short communication: Isolation and identification of actinomycetes potential as the antagonist of dickeya zeae pineapple soft rot in Lampung, Indonesia. Biodiversitas, 19(6): 2052–2058.

Bonaterra, A., E. Badosa, N. Daranas, J. Francés, G. Roselló and E. Montesinos. 2022. Bacteria as Biological control agents of plant diseases. Microorganisms, 10(9).

Boukhatem, Z. F., C. Merabet and H. Tsaki. 2022. Plant Growth Promoting Actinobacteria, the Most Promising Candidates as Bioinoculants. Frontiers in Agronomy,4: 12-21.

Chaiyasen, A., L. Chaiya, D.D. Douds and S. Lumyong. 2017. Influence of host plants and soil diluents on arbuscular mycorrhizal fungus propagation for on-farm inoculum production using leaf litter compost and agrowastes. Biological Agriculture and Horticulture, 33(1): 52–62.

Doolotkeldieva, T., M. Konurbaeva and S. Bobusheva. 2018. Microbial communities in pesticide-contaminated soils in Kyrgyzstan and bioremediation possibilities. Environmental Science and Pollution Research, 25(32): 31848–31862.

Goudjal, Y., M. Zamoum, N. Sabaou, F. Mathieu and A. Zitouni. 2016. Potential of endophytic Streptomyces spp. for biocontrol of Fusarium root rot disease and growth promotion of tomato seedlings. Biocontrol Science and Technology, 26(12): 1691–1705.

Haq, M. E., M.U. Shahbaz, M. Kamran, M.J. Matloob, W. Abrar, S. Ali and M.A. Iqbal. 2021. Relative potential of different plant extracts and antibiotics against Xanthomonas axonopodis pv. mangiferaeindicae causing bacterial leaf spot of mango in lab conditions. Pakistan Journal of Phytopathology, 33(2): 395-399.

Kamal, R., Y.S. Gusain and V. Kumar. 2014. Interaction and symbiosis of AM fungi, Actinomycetes and Plant Growth Promoting Rhizobacteria with plants: Strategies for the improvement of plants health and defense system. International Journal of Current Microbiology and Applied Sciences, 3(4): 21-33.

Kawuri, R., D. N. Suprapta and Y. Nitta. 2018. Antagonistic activity of Streptomyces thermocarboxydus to Fusarium oxysporum: The cause of leaf rot disease on Aloe Vera (Aloe barbadensis Mill.) in Bali, Indonesia. Malaysian Journal of Microbiology, 14(1): 10–15.

Klement, Z. and R. N. Goodman. 1967. The role of the living bacterial cell and induction time in hypersensitive reaction of tobacco plants. Phytopathology, 57: 322-323.

Kumar, A., S. Kumar Verma and D. K. Choudhary. 2014. Biochemical And Molecular Characterization Of Antagonistic Bacteria Against Yellow Blotch Of Oyster Mushroom. In IIT, BHU. India. 2 Ph.D. Scholar, School of Biochemical engineering. IIT, BHU. India. 4 Ph.D. Scholar. http://www.ijret.org

Lo Grasso, L., D. Chillura-Martino and R. Alduina. 2016. Production of Antibacterial Compounds from Actinomycetes. In Actinobacteria - Basics and Biotechnological Applications. Technology, 2; 15-26.

Murugan, A. V., T.P. Swarnam and S. Gnanasambandan. 2013. Status and effect of pesticide residues in soils under different land uses of Andaman Islands, India. Environmental Monitoring and Assessment, 185(10): 8135–8145.

Myo, E. M., B. Ge, J. Ma, H. Cui, B. Liu, L. Shi, M. Jiang and K. Zhang. 2019. Indole-3-acetic acid production by Streptomyces fradiae NKZ-259 and its formulation to enhance plant growth. BMC Microbiology, 19(1): 322-336.

Newman, M. M., N. Lorenz, N. Hoilett, N.R. Lee, R.P. Dick, M.R. Liles, C. Ramsier and J.W. Kloepper. 2016. Changes in rhizosphere bacterial gene expression following glyphosate treatment. Science of the Total Environment, 553: 32–41.

Nozari, R. M., F. Ortolan, L.V. Astarita and E.R. Santarém. 2021. Streptomyces spp. enhance vegetative growth of maize plants under saline stress. Brazilian Journal of Microbiology, 52(3): 1371–1383.

Nuria MC. 2010. Antibacterial Activities Jangkang (Homalocladium platycladium (F.Muell) Bailey) Leaves, Jurnal Mediagro, 2 (2): 9-15

Omran, R., and M.F. Kadhem. 2016. Production, purification, and characterization of bioactive metabolites produced from rare Actinobacteria Pseudonocardia Alni. Asian Journal of Pharmaceutical and Clinical Research, 9: 264–272.

Rabnawaz, M., G. Irshad, A. Majeed, M. Yousaf, R.A. Javaid, F. Siddique and A. Hanif. 2023. Trichoderma harzianum as growth stimulator and biological control agent against bacterial leaf blight (BLB) And blast of rice. Pakistan Journal of Phytopathology, 35(2): 317-326.

Rani, K., A. Dahiya, J.C. Masih and L. Wati. 2018. Actinobacterial Biofertilizers: An Alternative Strategy for Plant Growth Promotion. International Journal of Current Microbiology and Applied Sciences, 7(09): 607–614.

Raval, V., N. Shankar, S. Scsl and S. Ahmedabad. 2021. Isolation of microbes from valley of flower (vof) india and screening of actinomycetes for their antibiotic potential. Vol.3.

Robène, I., M. Perret, E. Jouen, A. Escalon, M.V. Maillot, A. Chabirand, A. Moreau, A. Laurent, F. Chiroleu and O. Pruvost. 2015. Development and validation of a real-time quantitative PCR assay to detect Xanthomonas axonopodis pv. allii from onion seed. Journal of Microbiological Methods, 114: 78–86.

Robène-Soustrade, I., D. Legrand, L. Gagnevin, F. Chiroleu, A. Laurent and O. Pruvost. 2010. Multiplex nested PCR for detection of Xanthomonas axonopodis pv. allii from onion seeds. Applied and Environmental Microbiology, 76(9): 2697–2703.

Roumagnac, P., O. Pruvost, F. Chiroleu and H. Hughes. 2004. Spatial ann temporal analysis of bacterial blight of onion caused by Xanthomonas axonopodis pv allii. Phytophatology, 94: 138– 146.

Salasia, S. I. O., Z. Khusnan, C. Lammler and M. Zschock. 2004. Comparative studies on pheno-and genotypic properties of Staphylococcus aureus isolated from bovine subclinical mastitis in central Java in Indonesia and Hesse in Germany. Journal of Veterinary Sciences, 5 (2): 103–109.

Sapkota, A., A. Thapa, A. Budhathoki, M. Sainju, P. Shrestha and S. Aryal. 2020. Isolation, Characterization, and Screening of Antimicrobial-Producing Actinomycetes from Soil Samples. International Journal of Microbiology, 2(3): 44-54.

Schaad, N. W., J.B. Jones and W. Chun. 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria, Third Edition. N. W. Schaad, J. B. Jones and W. Chun. 2001 Plant Pathology, 50(6): 812–814.

Schwartz, H. and D.H. Gent. 2006. Xanthomonas Leaf Blight of Onion. Colorado State University.

Selim, M., M. Selim, S.A. Abdelhamid and S.S. Mohamed. 2021. Secondary metabolites and biodiversity of actinomycetes. Journal of Genetic Engineering and Biotechnology, 19(72): 1–13.

Sharma, V. and R. Salwan. 2018. Biocontrol Potential and Applications of Actinobacteria in Agriculture. In New and Future Developments in Microbial Biotechnology and Bioengineering: Actinobacteria: Diversity and Biotechnological Applications, 2: 93–108.

Soumare, A., K. Boubekri, K. Lyamlouli, M. Hafidi, Y. Ouhdouch and L. Kouisni. 2021. Efficacy of phosphate solubilizing Actinobacteria to improve rock phosphate agronomic effectiveness and plant growth promotion. Rhizosphere, 17.

Yanti, Y. 2015. Peroxidase Enzyme Activity of Rhizobacteria-introduced Shallots Bulbs to Induce Resistance of Shallot towards Bacterial Leaf Blight (Xanthomonas Axonopodis pv allii). Procedia Chemistry, 14: 501–507.

Yanti, Y., H. Hamid and Nurbailis. 2023. Seminar Nasional Pariwisata dan Kewirausahaan (SNPK) Distribusi Penyakit Hawar Daun Bakteri Pada Tanaman Bawang Merah Di Sumatera. Seminar Nasional Pariwisata Dan Kewirausahaan (SNPK).

Yanti, Y., H. Hamid, Nurbailis and N.L. Suriani. 2022. Biological Activity of Indigenous Selected Plant Growth Promoting Rhizobacteria Isolates and their Ability to Improve the Growth Traits of Shallot (Allium ascalonicum L.). Philippine Journal of Science, 151(6): 2327–2340.

DOI: https://doi.org/10.33866/phytopathol.036.01.1096


  • There are currently no refbacks.

Copyright (c) 2024 Yulmira Yanti, Hasmiandy Hamid, Nurbailis Nurbailis, Yaherwandi Yaherwandi, Yenny Liswarni, Ilham Wibowo, Sisxri Selviana

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Pakistan Journal of Phytopathology
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.