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TOTAL PROTEIN POLYMORPHISM OF SOME ISOLATES OF FUSARIUM OXYSPORUM F. SP. ALBEDINIS USING SDS-PAGE
Abstract
Fusarium wilt caused by Fusarium oxysporum f. sp. albedinis (Foa), is one of the main diseases affecting date palm (Phoenix dactylifera L.), which poses a constant threat to palm oases, especially in North African countries. Since its appearance in 1870, this disease is responsible to the disappearance of more than 15 million palm trees, including three million of good quality date palm plantations in Algeria.
In this study, we performed electrophoresis of total proteins extracted from the mycelia of 20 Fusarium oxysporum f. sp. albedinis and 03 isolates of Fusarium sp. Protein was extracted and separated by SDS-PAGE electrophoresis followed by staining with silver nitrate. We noted variations in protein profile between the 23 Fusarium isolated from the three regions Ghardaïa, Bechar and Adrar.These results demonstrate the presence of important phenotypic polymorphism according to the ascending hierarchical classification of total proteins after the analysis of the phonograms constructed by the JMP software.
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DOI: https://doi.org/10.33866/phytopathol.033.01.0619
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Copyright (c) 2021 Abouamama Sidaoui, Noureddine Karkachi, Anis Bertella, Sadika Haouhacho, Samia Gharbi, Younes Elgoumiy, Ryme Terbeche, Mebrouk kihal
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