Yasir Ali, Azhar A. Khan, Muhammad Ijaz, Hafiz M. Aatif, Abdur Rahim, Salman Ahmad, Waseem Sabir, Saima Naseer, Muhammad Rafiq


The objective of present investigation was to investigate the thirty wheat advanced lines for characterization of environmental conditions conducive to leaf rust development. Moreover, eleven physio-morphological and yield attributing traits namely days to booting (DTB), days to heading (DTH), peduncle length (cm) plant height (cm), effective tillers, number of spikelet per spike, spike length, grains per spike, thousand-grain weight (g), grain yield (kg ha−1) and protein percentage were assessed on these genotypes in a Randomized Complete Block Design (RCBD) at the Research Area of Plant Pathology University College of Agriculture, B.Z.U. Bahadar Campus Layyah, to evaluate the elite advanced lines after clustering them based on their genetic variation in performance. Epidemiological variables showed a great influence on the progress of disease development. Minimum temperature (11-18.2 °C), maximum temperature (25-33 0C), relative humidity (44-64%), rainfall (2.2-9.1 mm) and wind speed (1.7-2.8 km/h) proved the most conducive factors for the development of leaf rust epidemics. Multivariate analysis exhibited that thirty advanced lines formed 4 different clusters. Cluster 2 and 3 showed maximum values for spike length, effective tillers, number of spikelet per spike, number of grains per spike, protein percentage, thousand-grain weight and yield and lowest peduncle length, days to booting and days to heading. Cluster 1 demonstrated maximum values for days to heading, days to booting, peduncle length and plant height and lowest for effective tillers, protein percentage, number of grains per spike, grain yield and thousand grain weight. Considering cluster analysis and yield contributing traits, members of cluster 2 may be used as high yielding and rust-resistant wheat elite lines in prevailing environmental conditions of district Layyah.


Cluster analysis, epidemiological variables, Genetic diversity, Puccinia recondite, PCA

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