Ahmad S. M. El-Nuby, Ashraf I. B. Afia, Eman A.S.A.H Alam


Ten marine algal extracts (MAE) viz. Amphiroa sp., Caulerpa racemosa, Dictyota sp., Halimeda discoidea, Jania sp., Laurencia obtusa, Padina pavonia, Sargassum latifolium, Sargassum polycystum, and Hormophysa cuneiformis; obtained by three solvents (water, methanol, and ethanol) were evaluated for their nematotoxicity against root-knot nematode (RKN) in vitro. Freshly hatched larvae of Meloidogyne incognita were exposed to these extracts. results revealed that; all examined MAE possessed nematotoxic properties with varing degrees. The highest mortality percentage (100 %) was detected in L. obtusa, Halimeda discoidea, and Padina pavonia, followed by Amphiroa sp. which recorded 95.00% mortality and not significantly different from Sargassum polycystum, and Dictyota sp. (89.00 and 89.30 %, respectively). Generally, methanol extract followed by ethanol was better than water regarding their nematicidal potential. Water was the less effective solvent to release anti-nematodal compounds from these studied algae, with the exception of Laurencia obtuse that able to kill 70.00 % of subjected juveniles. Preliminary phytochemical screening on all studied algal extracts revealed the presence of carbohydrates and/or glycosides, tannins, flavonoids, anthraquinones, steroids and/triterpenoids, sublimable substances, saponins, alkaloids and/or nitrogenous bases, cardiac glycosides, coumarins, chlorides, sulphates, and iridoids in all samples. In this regard, results of preliminary phytochemical screening showed that the richest extracts with these studied phytochemicals are belonging to methanolic extracts of these algae. The nematicidal action was related to the phytochemical structures of various marine algae and the extracting solvent. Phytochemical contents are positively correlated with antinematodal activity. These results offer promised bio-nematicide for combating phytonematodes by utilizing untapped marine algae. Evaluation of tested MAE under in vivo conditions will perform in the future to estimate their nematode-suppression, proper mode of application, and the significant dose. Future researches will focus on the preparation of some formulations for field application as biocides and growth promoters.


Phytochemicals, Preliminary Phytochemical Screening, Marine macroalgae, Nematotoxicity, Meloidogyne incognita.

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