Umara Arain, Mai J. Dars, Aziz A. Ujjan, Hadi B. Bozdar, Abdul Q. Rajput, Saleem Shahzad


Trichoderma harzianum is one of the most famous biocontrol agent in plant protection. Present study investigated the T. harzianum, either, to investigate there overall compatibility with commercial fungicide. The compatibility tests were conducted using the food poisoned technique adopted with dual culture methods. Among the seven commercial formulations of the fungicides T. harzianum strain was highly tolerable with the fungicide Thiovit Jet (Sulfur) with EC50 753.1 ppm followed by Ridomil Gold (Mancozeb 64, Metalex M 4 %) with EC50 469.1 ppm, Folio Gold (Metalexyl M 4% , Chlorothalonil 40%) @ EC50 227.8 ppm, it also tolerated to Kocide (Copper hydroxide) EC50 176.1 ppm, Tilt (Propiconazole) @ EC50 47.9 ppm, T. harzianum was highly susceptible to Score (Difenoconazole) @ EC50 13.2 ppm and highly Shincar (Carbendazim) @ EC50 2.5 ppm. Poisoned food media tests suggested that Kocide fungicide @ 50 ppm < EC10 synergized the antagonist potential of T. harzianum up to 03% against a pathogenic fungal isolate of F. oxysporum; Ridomil Gold and Folio Gold @ 150 and 70 ppm < EC10 synergized the biocontrol fungus against the pathogen in very slight values 1.0 to 0.5%, respectively; Thiovit jet showed negative effects on the biocontrol fungus; Shincar, Score and Tilt fungicides checked the T. harzianum growth. The in-vivo tritrophic tests are proposed to be examined using experimental designs for the combine utilizations of the treatments i.e. Soil + F. oxysporum + Kocide recommended doses + the strain of T. harzianum.


T. harzianum, fungicide synergism, fungicide compatibility, F. oxysporum

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