Gull e L. Khan, Gulshan Irshad, Farah Naz, Salman Ghuffar, Abdur R. Khalid, Shazia Arif, Amna Maqsood, Muhammad U. Raja, Raheem U. Din


Postharvest fungal rots are a continuous threat to perishable fruits worldwide. Fungal contamination is a significant factor affecting quality of peaches and resulting decline in their quantity. Postharvest health of peaches is of major concern for producers, consumers, marketers, and food industries globally. Early specie level detection of these deteriorating fungal rots is extremely important to adopt timely preventive measures and enhance shelf-life of peaches. In present study five fungal phytopathogenic rots were detected from peaches and identified based on molecular characterization using ITS universal fungal primers, EF-1α and β-tubulin (benA). Multi-locus characterization revealed fungal rots viz; Fusarium sporotrichioides, Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum and Cladosporium pseudocladosporioides respectively. Phylogenetic tree was constructed in MEGAX software using Neighbour- Joining Method and 1000 bootstrap replicates were selected to represent the evolutionary history of the taxa analyzed. Prior to management of various fungal rots, their accurate diagnosis is compulsory. Our results are significant in developing opportune control strategies from the field to storage hence reducing qualitative and quantitative losses and to enhance shelf-life of peaches.


Fusarium sporotrichioides; Aspergillus niger; Penicillium chrysogenum; Cladosporium pseudocladosporioides.

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Adaskaveg, J. E., H. Forster and D.F. Thompson. 2000. Identification and etiology of visible quiescent infections of Monilinia fructicola and Botrytis cinerea in sweet cherry fruit. Plant Disease, 3: 328-333.

Álvarez-Buylla, A., E. Culebras and J. J. Picazo. 2012. Identification of Acinetobacter species: is Bruker biotyper maldi-tof mass spectrometry a good alternative to molecular techniques. Infection Genetics and Evolution, 12(2): 345-349.

Bashar, M. A., S. Shamsi and M. Hossain. 2012. Fungi associated with rotten fruits in Dhaka Metropolis. Bangladesh Journal of Botany, 41(1): 115–117.

Capote, N., A.M. Pastrana, A. Aguado and P. Sánchez-Torres. 2012. Molecular tools for detection of plant pathogenic fungi and fungicide resistance. Plant Pathology, 12: 151-202.

Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39: 783-791.

Freeman, S., T. Katan and E. Shabi. 1998. Characterization of Colletotrichum species responsible for anthracnose diseases of various fruits. Plant Disease, 82(6): 596-605.

Kader, A. A. 2011. Postharvest biology and technology: An overview. In Postharvest Technology of Horticultural Crops,

Kumar, S., G. Stecher and K. Tamura. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7): 1870-1874.

Li, M. X., C. F. Liu, R. Wang, F. Y. Zheng, J. J. Wang, C. T. Niu, Q. Li. 2021. Optimization of Fermentation Process and Analysis of Aroma Components of Yellow Peach Wine. Journal Food Science Biotechnology, 40: 39–49.

Moss, M.O. 2002. Mycotoxin review 1 Aspergillus and Penicillium. Mycologist, 16: 116-119.

Narayanasamy, P. 2011. Detection of fungal pathogens in plants. In Microbial PlantPathogens-Detection and Disease Diagnosis. Springer, 11: 23-44.

Parfitt, J., M. Barthel and S. Macnaughton. 2010. Food waste within food supply chains: Quantification and potential for change to 2050. Philosophy Transitional Research Society, 365: 3065–3081.

Parpia, H. 1976. Post-harvest impact of their prevention on food supplies, nutrition and development. In: Symposium on Nutrition and Agricultural and Economic Development in the Tropics. Plenun Press New York, 21: 195–206.

Pscheidt, J. W. and C. M. Ocamb. 2019. Pear (Pyrus spp.) Storage rots. Pacific Northwest Plant Disease Management Handbook. Oregon State University, Corvallis.

Rehman, Z. U., S. F. Faisal, S. Humayun and A.B. Muhammad. 2020. Peach Rot Disease Prevalence in the Markets of Federal Capital Territory, Pakistan. Pakistan Journal of Agricultural Research, 33: 429.

Saitou, N., and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4: 406-425.

Spadaro, D. and S. Droby. 2016. Development of biocontrol products for postharvest diseases of fruit: The importance of elucidating the mechanisms of action of yeast antagonists. Trends in Food Science and Technology, 47: 39–49.

Stecher, G., K. Tamura and S. Kumar. 2020. Molecular Evolutionary Genetics Analysis (MEGA) for macOS. Molecular Biology and Evolution, 37: 1237-1239.

Sugar, D. 2002. Management of postharvest diseases. Fruit Quality and Its Biological Basis. Sheffield Academic Press, 21: 225-252.

Tamura, K., G. Stecher and S. Kumar. 2021. MEGA 11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution,

Tamura, K., M. Nei, M and S. Kumar. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA), 101:11030-11035.

Usall, J., C. Casals, M. Sisquella, L. Palou and A. de Cal. 2015. Alternative technologies to control postharvest diseases of stone fruits. Stewart Postharvest Review, 11: 1–6.

White, T. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. MA Innis et al., eds. In: Academic Press, San Diego, CA,

Zhao, X., W. Zhang, X. Yin, M. Su, C. Sun, X. Li and K. Chen. 2015. Phenolic composition and antioxidant properties of different peach Prunus persica (L.) Batsch cultivars in China. International Journal of Molecular Sciences, 16(3): 5762-5778.



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Pakistan Journal of Phytopathology
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