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CHARACTERIZATION AND REPORT OF ALTERNARIA ALTERNATA CAUSING LEAF SPOT DISEASE IN PLUM TREES IN KARBALA IRAQ
Abstract
In the growth season of 2018-2019, plum trees in many orchards in the Al-Hussainiya district of Karbala Province, Iraq, regularly exhibited severe symptoms of leaf spot. The prevalence of the disease was estimated to be 15%. The causal factor responsible for this disease has been determined as Alternaria alternata based on its morphological and molecular characteristics. The pathogenicity of the isolated A. alternata was assessed using Koch's postulates, which confirmed its pathogenic nature. Therefore, it was once again isolated from the affected symptomatic leaves. The results outline the scientific methodology used to identify the causal agent responsible for the Alternaria leaf spot disease on plum trees. This is the first report of A. alternata causing leaf spot on plum trees in Karbala province, Iraq.
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Abdulmoohsin, R. G., A.A. Lahuf, Y.N. Husain and Z.L. Hameed. 2019. Bio-efficiency of some indigenous biocontrol agents against Rhizoctonia solani causing cowpea seed rot and preemergence damping-off. IOP Conf. Ser.: Earth and Environmental Sciences, 388. 012011.doi:10.1088/1755-1315/388/1/012011.
Almousawy, M. A., A.A. Lahuf and O. Hade. 2017. Isolation and diagnosis of the pathogens causing seed decay and damping-off disease on wheat and control them using some biological and chemical factors. Journal of Kerbala for Agricultural Sciences, 4(1): 112–132.
Al-Tememe, Z.A.M., A. Lahuf, R.G. Abdalmoohsin and A.T. Al-Amirry. 2019 Occurrence, identification, pathogenicity and control of Neoscytalidium dimidiatum fungus, the causal agent of sooty canker on Eucalyptus camaldulensis in Kerbala Province of Iraq. Plant Archive, 19: 31–38.
Bortiri, E., H.B. Vanden and D. Potter. 2006. Phylogenetic analysis of morphology in Prunus reveals extensive homoplasy. Plant Systematic and Evolution, 259: 53–71.
Carbone, I. and L.M. Kohn. 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycology, 91:553–556.
Ellis, M.B. 1971. Dematiaceous Hyphomycetes, Commonwealth Mycological Institute, Kew, UK.
Food and Agriculture Organization of the United Nations. 2023. FAOSTAT [Database]. Retrieved from http://www.fao.org/faostat/en/#data
Hameed, Z. L., A.A. Lahuf, M.T. Jasim, H.M. Mohsen, B.J. Kadim, S.A. Saleh and A.F. Mohamed. 2021. First Report of Alternaria alternata causing brown leaf spot on apricot (Prunus Armeniaca) in Karbala Province of Iraqâ€. Earth and Environmental Sciences, 910: 012080â€.
Hussein, M.A. and K. Voigt, 2019. Phylogenetic and enzymatic variability of Alternaria species isolated from various substrates in Qena governorate of Upper Egypt. Achieves of Phytopathology and Plant Protection, 52: 530–541.
Jaber, M.H. and A.A. Lahuf. 2020. Survey, pathogenicity and molecular identification of novel Fusarium species causing seed decay and damping-off disease of wheat crop in Kerbala Province, Iraq. Plant Cell Biotechnology and Molecular Biology, 21: 1–14.
Kole, C. and A.G. Abbott. 2012. Genetics, genomics and breeding of stone fruits. CRC Press,Taylor & Francis Group, 6000 Broken Sound Parkway NW, Florida-USA.
Lahuf A.A., O.H. Jaafar, M. Al-mosoy and Z.L. Hameed. 2018a. First record of the crown rot fungus Fusarium equiseti affecting Triticum aestivum L. and Aptenia cordifolia in Iraqâ€. Asian Journal of Agriculture and Biology,6 (4): 543-548.â€
Lahuf, A. A., R.A.G. Abdalmoohsin, A.H. Alhusani, and A. Al-Asadi. 2018b. First report of leaf blight disease in lily (Lilium candidum) caused by Alternaria alternata in Iraqâ€. Biopesticides International, 14 (2): 123-126â€.
Lahuf, A. A. 2019a. Alternaria alternata causes leaf blight of rosy periwinkle (Catharanthus roseus) in Iraq. Australasian Plant Disease Notes, 14(4):1-4.
Lahuf, A.A. 2019b. First report of Fusarium proliferatum causing stem and root rot on lucky bamboo (Dracaena braunii) in Iraq, Hell. Plant Protection Journal, 12: 1–5.
Lahuf, A. A, O.H. Jaafar and Z.L. Hameed. 2019. A simple, rapid, safe and low-cost method to extract DNA from phytopathogenic fungi. Asian Journal of Agriculture and Biology. 7(2):197-203.
Lahuf, A. A, K.M. Abdullah and M.T. Mohammadali. 2020a. Assessment of the nano sized particles of ZnO and MgO and some cultivars in control of Alternaria solani causing tomato early blight. Ecology, Environment and Conservation Journal, 26: 89-95.
Lahuf, A. A., Kareem, A.A., Mohammadali, M.T., Mohsen, H.M., Li, J. 2020b. First report of Alternaria alternata causing leaf spot on quince (Cydonia oblonga) in Iraq. Plant Disease,104 (5): 1538-1538.
Lahuf, A. A, A.Q. Kadhim, Z.L. Hameed and A.M.N. Almulla. 2022. First report of Paecilomyces rot on rose (Rosa sp.) in Iraq. Journal of Plant Pathology, 104:1195.
Long, H., S. Yang, X. Yin, Z. Zhao, Y. Long, J. Fan, R. Shu and G. Gu. 2021. First report of leaf spot on Prunus salicina caused by Alternaria alternata in China. Journal of Plant Pathology, 103:1021
Lozano, M., V.M. Carmen, H.M. Teresa, A.M. Concepcion, B.M. Josefa and J.V.B. Garcia. 2009. Physicochemical and nutritional properties and volatile constituents of six Japanese plum (Prunus salicina Lindl.) cultivars. European Food Research and Technology, 228: 403–410.
Meena, M., S.K. Gupta, P. Swapnil, A. Zehra, M.K. Dubey and R.S. Upadhyay. 2017. Alternaria toxins: Potential virulence factors and genes related to pathogenesis. Frontiers in Microbiology, 8: 1–14.
Okie, W.R. and J.F. Hancock. 2008. Plums. In: Hancock JF (ed) Temperate Fruit Crop Breeding: Germplasm to Genomics. Springer, Berlin, Heidelberg, New York, pp. 337–358.
Parkunan, V., S. Li, E.G. Fonsah and P. Ji. 2013. First report of Alternaria leaf spot of banana caused by Alternaria alternata in the United States. Plant Disease. 97(8):1116.
Pettitta, T.R., M.F. Wainwrighta, A.J. Wakehamb and J.G. White. 2011. A simple detached leaf assay provides rapid and inexpensive determination of pathogenicity of Pythium isolates to ‘all year round’ (AYR) chrysanthemum roots. Plant Pathology, 60: 946–956.
Pinto, V.E.F. and A. Patriarca. 2017. Alternaria species, and their associated mycotoxins. In Mycotoxigenic Fungi: Methods in Molecular Biology; Moretti, A., Susca, A., Eds.; Humana Press: New York, NY, USA; Volume 1542, pp. 13–32.
Ramezani, Y., P. Taheri and M. Mamarabadi. 2019.Identification of Alternaria spp. associated with tomato early blight in Iran and investigating some of their virulence factors. Journal of Plant Pathology, 101: 647–659.
Saleem, A. and A.A. El-Shahir. 2022. Morphological and molecular characterization of some Alternaria species isolated from tomato fruits concerning mycotoxin production and polyketide synthase genes. Plants, 11(1168):1-13.
Sanders, S.W. 1993. Dried Plums: A multi-functional bakery ingredient. American Society of Bakery Engineers; Bulletin 228, pp. 1-23.
Simmons, E.G. 2007. Alternaria: an identification manual. Utrecht, Netherland: CBS Fungal biodiversity centre.
Tamura, K., G. Stecher, D. Peterson, A. Filipski and S. Kumar. 2013. MEGA6: Molecular Evolutionary Genetics Analysis, version 6th ed. Molecular Biology Evolution 30: 2725–2729.
Voca, S., A. Galic, Z. Sindrak, N. Dobricevic, S. Pliestic and J. Druzic. 2009. Chemical composition and antioxidant capacity of three plum cultivars. Agriculturae conspectus scientificus, 74: 273–276.
White, T.J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR Protocols: A Guide to Methods and Applications. San Diego, CA, USA: Academic Press, 315-322.
Woudenberg, J.H.C., M.F. Seidl, M.F, J.Z. Groenewald, M. de Vries, J.B. Stielow, B.P.H.J. Thomma and P.W. Crous. 2015. Alternaria section Alternaria: Species, formae speciales or pathotypes. Studies in Mycology, 82: 1–21.
DOI: https://doi.org/10.33866/phytopathol.036.01.0918
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