Muhammad H. Hassan, Saif Ullah, Asim Mehmood, Aqsa Amin, Muhammad U. Shahzad


The quality characteristics of Oyster mushrooms (Pleurotus spp.) under various environmental conditions and substrate compositions are thoroughly evaluated in this study. The objective of this successful research was to focus on investigating the role of synergistic relation of temperature ranges of 10-15°C, 15-25°C, and 25-35°C, along with humidity levels of 65% and 85% on various substrates named Substrate A, a combination of wheat straw and sawdust, cardboard, leaves, wheat straw, and sawdust along with the addition of 25 g of Plant fibers, lime and chicken dung in all substrates. The key parameters including First Primordial Initiation (DPI), Number of Effective Fruiting Bodies (NFB), Biological Efficiency (BE), and Yield (in grams) per 500g of substrate dry weight, Stem Diameter, Total Days of cultivation, were employed to check mushroom development. Overall, findings reveal that the optimal conditions for Oyster Mushroom growth are observed at 15-25°C with 85% relative humidity, resulting in the highest Biological Efficiency (BE) at 56% on Substrate A as compared to other environmental ranges, but 65 % RH gives more yield with higher temperatures at 25-35°C., All substrates except leaves give better yield at 85% RH rather than 65%. Leaves give the better yield on 65% RH as at 249g. Substrate A, a combination of wheat straw and sawdust, yielded the most favorable outcomes, exhibiting an average DPI of 7, NFB of 50, and a yield of 375g, with a remarkable BE of 75%. Moreover, the hypothesis is proved that a combination of substrates can provide more yield and results demonstrate that a combination can give more 17.9 % yield. The best substrates in terms of all quality indicators are cardboard and substrate A (wheat straw plus sawdust). This research recommends the Cardboard while focusing on the best Diameter of the Stem as the greatest diameter of the stem in cardboard is 53 but substrate A is the best overall for all factors. It is also recommended to use the combination of the substrates just like substrate A for fast growth because substrate A has less TDM in all conditions. So, this study provides valuable insights into optimizing mushroom cultivation practices by strategically selecting suitable substrates and environmental settings to enhance both yield and quality.


Oyster Mushrooms; Substrate Composition; Quality parameters; Mushroom Cultivation; Environmental Conditions.

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Bao, D., S. Kinugasa and Y. Kitamoto. 2004. The biological species of oyster mushrooms (Pleurotus spp.) from Asia based on mating compatibility tests. Journal of Wood Science, 50, 162-168.

BISWAS, S., M. Datta and S. Ngachan. 2011. Mushrooms: A Manual for cultivation: PHI Learning Pvt. Ltd.

Bret, J. M. 1987. Environmental factors affecting growth. In: Hoare, W. H., D. J. Randall, S. R. Bret (Eds.), Fish Physiology, Academic Press, 8:252–259.

Gumede, T. 2008. Effects of prolonged soaking on the elimination of contaminants. Botswana University of Agricultural and Natural Resources. A Project Report, 23-30.

Haimid, M. T., H. Rahim and R. A. Dardak. 2013. Understanding the mushroom industry and its marketing strategies for fresh produce in Malaysia. Economic and Technology Management Review, 8:27-37.

Hawksworth, D. L. 2001. Mushrooms: the extent of the unexplored potential. International Journal of Medicinal Mushrooms, 3(4).

Ibrahim, R., L. S. Boon, M. N. I. H. Mazidi and N. D. Yaacob. 2020. Effects of electrical shock and blue LED treatments on the growth, yield and quality of grey oyster mushrooms (Pleurotus sajor-caju). Paper presented at the IOP Conference Series: Materials Science and Engineering.

Jongman, M., K. B. Khare and D. Loeto. 2018. Oyster mushrrom cultivation at different production systems: A. European Journal of Biomedical and Pharmaceutical Sciences, 5(5), 72-79.

Jour, Mago, Payal, Gunwal, Isha 2014. Commercial production of Oyster mushroom. IOSR Journal of Environmental Science, Toxicology and Food Technology, 8.

Jo, W. S., Y. J. Cho, D. H. Cho, S. D. Park, Y. B. Yoo and S. J. Seok. 2009. Culture conditions for the mycelial growth of Ganoderma applanatum. Mycobiology, 37(2): 94-102.

Khonga, E., K. Khare and M. Jongman. 2013. Effect of different grain spawns and substrate sterilization methods on yield of oyster mushroom in Botswana. International Journal of Bioassays, 2(10):1308-1311.

Mandeel, Q., A. Al-Laith and S. Mohamed. 2005. Cultivation of oyster mushrooms (Pleurotus spp.) on various lignocellulosic wastes. World Journal of Microbiology and Biotechnology, 21:601-607.

Nongthombam, J., A. Kumar, B. Ladli, M. Madhushekhar and S. Patidar. 2021. A review on study of growth and cultivation of oyster mushroom. Plant Cell Biotechnology and Molecular Biology, 22(5-6):55-65.

Numaguchi, K. 1994(a). Growth and physiological condition of the Japanese pearl oyster, Pinctada fucata martensii (Dunker, 1850) in Ohmura Bay, Japan. Journal of Shellfish Research, 13:93–99.

Numaguchi, K. 1994(b). Effect of water temperature on the filtration rate of Japanese pearl oyster, Pinctada fucata martensii. Journal of the Japanese Aquaculture Society, 42:1–6 (in Japanese with English abstract).

Numaguchi, K. and Y. Tanaka. 1986(a). Effects of salinity on mortality and growth of the spat of the pearl oyster, Pinctada fucata martensii. Bulletin of National Research Institute of Aquaculture, 9:41–44.

Patel, Y., R. Naraian and V. Singh. 2012. Medicinal properties of Pleurotus species (oyster mushroom): a review. World Journal of Fungal and Plant Biology, 3(1):1-12.

Randive, S. D. 2012. Cultivation and study of growth of oyster mushroom on different agricultural waste substrate and its nutrient analysis. Advances in Applied Science Research, 3(4):1938-1949.

Senatore, W. S. D. B. P. and M. A. K. G. S. Soriente. 2004. Identification and characterization of Trichoderma species aggressive to Pleurotus in Italy. 30(4): 469-470.

Uemoto, H. 1968. Relationship between oxygen consumption by the pearl oyster and its environmental temperature. Bulletin of the National Pearl Research Laboratory, 13:1617–1623.

Utami, C. P. and P. R. Susilawati. 2017. Rice straw addition as sawdust substitution in oyster mushroom (Pleurotus ostreatus) planted media. Paper presented at the AIP Conference Proceedings.

Wada, K. 1991. The pearl oyster, Pinctada (Gould) (family Pteriidae). In: Menzel, W. (Ed.), Estuarine and Marine Bivalve Mollusk Culture. CRC Press, Boston, pp. 245–260.



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Pakistan Journal of Phytopathology
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