IN-VITRO EVALUATION OF HOMEO-FUNGICIDES AND METHANOLIC PLANT EXTRACTS AGAINST MYCELLIAL GROWTH OF FUSARIUM OXYSPORUM F.SP. PISI CAUSING WILT DISEASE IN PEA

Abstract

Fungitoxic effects of three homeo-fungicides viz; Rigrous, Ventage, Vampire and six methanolic plant extracts viz; Garlic Extract, Ginger Extract, Akk Leaf Extract, Neem Leaf Extract, Moringa Leaf Extract and Parthenium Leaf Extract at nine different concentrations viz; 5, 10, 20, 50, 100, 150, 300, 500 and 1000 µg/ml were tested in vitro by applying poisoned food technique method at an incubation period of nine days at 25 ± 2°C. Mycellial growth of the fungus in response to various homeo-fungicidal at different concentrations was compared Vampire proved to be the best as it has reduced the mycelial colony (83.4 percent) which was followed by Rigrous (82.2 percent), while Vantage proved to be the least effective (76.5 percent) at highest concentration (1000 µg/ml). None of the other homeo-fungicides have completely checked the mycelial growth of F. oxysporum f.sp. pisi. The experiment was arranged in randomized complete design (CRD) However, when growth of the fungus in response to various methanolic plant extracts and their concentrations were compared, Neem leaf extract proved to be the best as it had given the maximum colony reduction (60.5 percent) followed by Ginger extract (57.7 percent), while Parthenium leaf extract proved to be the least effective (25.4 percent) at highest concentration (1000 µg/ml). None of the tested Plant extracts and their concentrations tested has completely checked the mycelial growth. There was an overall trend of reduction in mycellial growth of fungus with an increase in the concentration of homeo-fungicides and methanollic plant extracts.