Protecting The Future of Citrus: A Mini Review On Citrus Canker Threats, Control Strategies, And Future Outlooks
DOI:
https://doi.org/10.33866/phytopathol.037.01.1213Keywords:
citrus canker, Xanthomonas axonopodis pv. Citri, integrated disease management, citrus leaf miner, induced systemic resistance.Abstract
Citrus canker, caused by Xanthomonas citri subsp. citri (previously known as Xanthomonas axonopodis pv. citri) is a devastating disease impacting citrus production globally. This review outlines the pathogen’s origin, epidemiology, and spread, highlighting its severe economic impact, particularly in regions like Pakistan. The interaction between environmental factors and citrus leaf miners exacerbates disease severity, causing significant yield losses. Traditionally, citrus canker management has relied on synthetic chemicals such as copper-based bactericides, but their overuse has led to environmental concerns and pathogen resistance. Sustainable alternatives, including essential oils, are being explored for disease control. Recent studies demonstrate the potential of clove, cinnamon, and neem oils, with clove oil showing the highest antibacterial efficacy against X. citri. Integrated disease management strategies, incorporating quarantine measures, resistant cultivars, and plant extracts, are discussed as promising approaches to mitigate the spread and severity of citrus canker, with particular attention to eco-friendly options like induced systemic resistance and essential oils. However, gaps remain in understanding these alternatives' full efficacy and application methods, underscoring the need for further investigation. The review concludes with recommendations for future research into eco-friendly solutions, such as induced systemic resistance and essential oils, to reduce dependence on harmful chemicals and enhance citrus crop resilience.References
Ali F., M. Atiq, N.A. Rajput, I. Ahmad, M.A. Latif, M.A. Aslam, M.J. Matloob, M. Mehtab, A. Ijaz and M. Qasim. 2024. Intervention of Bacterial Leaf Spot of Bell Pepper through Neem Mediated Copper and Zinc Hybrid Nanoparticles. Phytopathogenomics and Disease Control, 3(2): 251-259.
Ali, A., F. Ölmez, M.A. Zeshan, M. Mubeen, Y. Iftikhar, A. Sajid, and M.K. Solanki. 2024c. Yeast-Based Solutions in Controlling Plant Pathogens. Biocatalysis and Agricultural Biotechnology, 103199. https://doi.org/10.1016/j.bcab.2024.103199
Ali, A., I. Tabbasum, H. Azeem, F. Ölmez, G. Deveci, B. Khalid, and M. Mehtab. 2023a. Bacterial endophytes, a resilient way toward sustainable agriculture: provide plant growth promotion and biocontrol of plant pathogens. Journal of Global Innovations in Agricultural Sciences, 11(2): 153–174
Ali, A., M. Aasim, K. Çelik, M.A. Nadeem, and F.S. Baloch. 2024a. Frontiers in bacterial-based green synthesized nanoparticles (NPs): A sustainable strategy for combating ınfectious plant pathogens. Biocatalysis and Agricultural Biotechnology, 68 https://doi.org/10.1016/j.bcab.2024.103293
Ali, A., M. Shahbaz, F. Ölmez, N. Fatima, U.U.D. Umar, M.A. Ali, and F.S. Baloch. 2024b. RNA interference: a promising biotechnological approach to combat plant pathogens, mechanism and future prospects. World Journal of Microbiology and Biotechnology, 40(11):339
Ali, A., M.A. Zeshan, Y. Iftikhar, M. Abid, S.F. Ehsan, M.U. Ghani, and A.A. Khan. 2020. Role of plant extracts and salicylic acid for the management of chili veinal mottle virus disease. Pakistan Journal of Phytopathology, 32(2), 147–157
Ali, A., U.U.D. Umar, S. Akhtar, M.T. Shakeel, A.U. Rehman, M.N. Tahir, S. Atta, M. Moustafa, F. Ölmez, and R. Parveen. 2022b. Identification and primary distribution of Citrus viroid V in citrus in Punjab, Pakistan. Molecular Biology Reports, 49(12): 11433–11441 https://doi.org/10.1007/s11033-022-07677-7
Ali, A., U.U.D. Umar, S.A.H. Naqvi, M.T. Shakeel, M.N. Tahir, M.F. Khan, M.T. Altaf, F. Ölmez, A.A. Dababat, Z.U. Haq, M.A. Nadeem, R. Hatipoğlu, F.S. Baloch, and Y.S. Chung. 2023b. Molecular characterization of divergent isolates of Citrus bent leaf viroid (CBLVd) from citrus cultivars of Punjab, Pakistan. Frontiers in Genetics, 13https://doi.org/10.3389/fgene.2022.1104635
Ali, A., Y. Iftikhar, M. Mubeen, H. Ali, M.A. Zeshan, Z. Asad, M. Zafar-ul-Hye, M.A. Rehman, M. Abbas, M. Rafique and M.U. Ghani. 2022a. The antagonistic potential of bacterial species against fungal plant pathogens (FPP) and their role in plant growth promotion (PGP): A review. Phyton-International Journal of Experimental Botany, 91(9): 1859–1877
Ali, S., A. Hameed, G. Muhae-Ud-Din, M. Ikhlaq, M. Ashfaq, M. Atiq, F. Ali, Z.U. Zia, S.A.H. Naqvi and Y. Wang. 2023c. Citrus canker: A persistent threat to the worldwide citrus industry—an analysis. Agronomy, 13(4): 11–12
Aslam N., M. Atiq, N.A. Rajput, A. Akram, A.M. Arif, G.A. Kachelo, A. Nawaz, M.M. Jahangir, A. Jabbar and A. Ijaz. 2024. Explicating botanical bactericides as an intervention tool towards citrus canker. Plant protection, 08(01): 25-32.
Ayilara, M.S., B.S. Adeleke, S.A. Akinola, C.A. Fayose, U.T. Adeyemi, L.A. Gbadegesin, R.K. Omole, R.M. Johnson, Q.O. Uthman, and O.O. Babalola. 2023. Biopesticides as a promising alternative to synthetic pesticides: A case for microbial pesticides, phytopesticides, and nanobiopesticides. Frontiers in Microbiology,14 https://doi.org/10.3389/fmicb.2023.1040901
Azeem, H., A. Ali, R. Perveen, U.U.D. Umar, M. Zakaria, F. Ölmez, and M.U. Azeem. 2024. Molecular characterization of cotton leaf curl Multan beta-satellite occurance on chili plants in Multan, Pakistan. Journal of Plant Protection Research, 64(2):139-148 https://doi.org/10.24425/jppr.2024.150248
Behlau, F. 2021. An overview of citrus canker in Brazil. Tropical Plant Pathology, 46(1): 1–12.
Behlau, F., N.L. Barelli, and J. Belasque. 2014. Lessons from a case of successful eradication of citrus canker in a citrus-producing farm in São Paulo state, Brazil. Journal of Plant Pathology, 96(3): 561–568
Bhatti, A.M., H.M. Usman, A. Iffat, M. Tatar, M.M. Karim, M.I. Zafar, A. Ali and T. Shafique. 2024. Deciphering the Current Scenario and Prospective Outlook of Citrus Gummosis in Pakistan. Düzce Üniversitesi Ziraat Fakültesi Dergisi, 2(1): 46–59
Caglayan, K., M. Gazel, and D. Škorić. 2019. Transmission of phytoplasmas by agronomic practices. In Assunta Bertaccini, Phyllis G Weintraub, Govind Pratap Rao, Nicola Mori (Eds.), Phytoplasmas: Plant pathogenic bacteria-II: Transmission and management of phytoplasma-associated diseases (pp. 149–163)
Caicedo, J.C., & S. Villamizar. 2021. Xanthomonas citri ssp. citri pathogenicity, a review. In M. S. Khan & I. A. Khan (Eds.), Citrus-research, development and biotechnology.
Castillo-Juárez, I., B.E. Blancas-Luciano, R. García-Contreras, and A.M. Fernández-Presas. 2022. Antimicrobial peptides properties beyond growth inhibition and bacterial killing. PeerJ: Life and Enviroment, 10: Article e12667. https://doi.org/10.7717/peerj.12667
Cheema, I.A. and H.K. Jamali. 2020. Growth of citrus fruits in Pakistan. Amazonia Investiga, 9(35): 74–81. https://doi.org/10.34069/AI/2020.35.11.6
Cheema, I.A., H.K. Jamali, and A. Sarwar. 2020. Pakistan citrus exports break record in 2020–21 season. Trade Development Authority of Pakistan.
Chuang, S.C., S. Dobhal, K. Pal, T.D. Amore, A.M. Alvarez, and M. Arif. 2024. Xanthomonas Strains Isolated from Hosts in the Family Araceae Reveal Diverse Phylogenetic Relationships and Origins. Phytopathology, 114(8), 1963–1974
Constantin, E.C., A. Haegeman, J. Van Vaerenbergh, S. Baeyen, C. Van Malderghem, M. Maes, and B. Cottyn. 2017. Pathogenicity and virulence gene content of Xanthomonas strains infecting Araceae, formerly known as Xanthomonas axonopodis pv. dieffenbachiae. Plant Pathology, 66(9): 1539–1554 https://doi.org/10.1111/ppa.12694
Das, A.K. (2003). Citrus canker: A review. Journal of Applied Horticulture, 5(1): 52–60
Dhiman, M.R., R. Kumar, and S. Kumar. 2021. Postharvest Handling and Disease Management of Cut Flowers. In D. Singh, R.R. Sharma, V. Devappa, D. Kamil (Eds.), Postharvest handling and diseases of horticultural produce (pp. 415–430).
Dia, N.C., L. Morinière, B. Cottyn, E. Bernal, J.M. Jacobs, R. Koebnik, E. Osdaghi, N. Potnis and J.F. Pothier. 2022. Xanthomonas hortorum – beyond gardens: Current taxonomy, genomics, and virulence repertoires. Molecular Plant Pathology, 23(5): 597–621 https://doi.org/10.1111/mpp.13185
Ference, C.M., A.M. Gochez, F. Behlau, N. Wang, J.H. Graham and J.B. Jones. 2018. Recent advances in the understanding of Xanthomonas citri ssp. citri pathogenesis and citrus canker disease management. Molecular Plant Pathology, 19(6): 1302–1318 https://doi.org/10.1111/mpp.12638
Gabriel, D.W., M.T. Kingsley, J.E. Hunter, and T. Gottwald. 1989. Reinstatement of Xanthomonas citri (ex-Hasse) and X. phaseoli (ex-Smith) to species and reclassification of all X. campestris pv. citri strains. International Journal of Systematic and Evolutionary Microbiology, 39(1): 14–22
Gmitter, F.G., C. Chen, M.A. Machado, A.A. De Souza, P. Ollitrault, Y. Froehlicher, and T. Shimizu. 2012. Citrus genomics. Tree Genetics & Genomes, 8: 611–626
Goto, M. (2012). Fundamentals of bacterial plant pathology.
Graham, J.H., E.G. Johnson, M.E. Myers, M. Young, P. Rajasekaran, S. Das, and S. Santra. 2016. Potential of nano-formulated zinc oxide for control of citrus canker on grapefruit trees. Plant disease, 100(12): 2442–2447. https://doi.org/10.1094/PDIS-05-16-0598-RE
Graham, J.H., T.R. Gottwald, J. Cubero, and D.S. Achor. 2004. Xanthomonas axonopodis pv. citri: factors affecting successful eradication of citrus canker. Molecular Plant Pathology, 5(1): 1–15
Guarnaccia, V., J.Z. Groenewald, G. Polizzi, and P.W. Crous. 2017. High species diversity in Colletotrichum associated with citrus diseases in Europe. Persoonia-Molecular Phylogeny and Evolution of Fungi, 39(1): 32–50 https://doi.org/10.3767/persoonia.2017.39.02
Hameed, A., N.A. Rajput, R. Binyamin, F. Ali, M. W. Alam, H. M. U. Aslam, H. Riaz, and S. Ali. 2023. Epidemiological Markers for Citrus Canker caused by Xanthomonas citri pv. citri. International Journal of Phytopathology, 12(3): 295–302
Haq ZU, A. Ali, S. A. H. Naqvi, F. S. Baloch, and U. U. D. Umar. 2024. Primary distribution and RT-PCR based detection of citrus dwarfing viroid (CDVd) in citrus cultivars of Pakistan. Turkish Journal of Botany 48: 1-11. https://doi.org/10.TJBOT-2024-00200R1.
Hughes, T., J. Dall'Asta, and S. Turner. 2022. Integrated Disease Management and its impact on young seedling cases of citrus canker. Journal of Integrated Pest Management, 13(1): 19–27. https://doi.org/10.1093/jipm/pmac015
Iftikhar, Y., M. Mubeen, Q. Shakeel, I. Lalarukh, A. Ali, M. Aasim, M.A. Zeshan, T. Zulfiqar, S. K. Alhag, and L.A. Al-Shuraym. 2024. Spatial distribution and molecular characterization of huanglongbing and its vector in various citrus cultivars. Pakistan Journal of Agricultural Sciences, 61(2): 685–694 https://doi.org/10.21162/PAKJAS/24.155
Jabeen, T., H.M.I. Arshad, K. Saleem, S. Ali, E. Ullah, S. Naureen and M.M. Babar. 2016. Morphological and biochemical characterization of Xanthomonas axenopodis pv. citri isolates causing citrus canker disease in Pakistan. PSM Microbiology, 1(1), 10–17
Jamil, Z., M. Tariq, M.U. Raja, M. Shahbaz, J.S.S. Seelan, M. Tatar, A. Ali, N. Fatima, R. Bejaoui, and A.U. Bozhüyük. 2024. Utilization of phytogenic silver nanoparticles and bacterial agents for the management of citrus canker disease. Turkish Journal of Agriculture and Forestry, 48: 1-13
Jyothi, V., and M.E. Shilpa. 2020. Citrus Diseases and Their Management. In G. Chand, N. Akhtar and S. Kumar (Eds.), Diseases of Fruits and Vegetable Crops (pp. 41–63).
Kebede, M., and K. Bamud. 2023. Assessment of major fruit diseases and insect pests in silte, gurage and yem special woreda, southern Ethiopia. Ukrainian Journal of Ecology, 13(3): 20–26 https://doi.org/10.15421/2023_435
Khan, A., M. Ikram, J.R. Hahm, and M.O. Kim. 2020. Antioxidant and anti-inflammatory effects of citrus flavonoid hesperetin: Special focus on neurological disorders. Antioxidants, 9(7). https://doi.org/10.3390/antiox9070609
Khan, M.A. and M. Abid. 2007. Effect of environmental conditions on citrus canker disease development. Pakistan Journal of Phytopathology, 19(2): 139–144
Leduc, A., C. Vernière, C. Boyer, K. Vital, O. Pruvost, Y. Niang and J.Y. Rey. 2011. First report of Xanthomonas citri pv. citri pathotype A causing Asiatic citrus canker on grapefruit and Mexican lime in Senegal. Plant Disease, 95(10): 1311–1311 https://doi.org/10.1094/PDIS-03-11-0217
Lee, R.F. 2020. Exclusion of plant viruses by certification and quarantine programs. In L.P. Awasthi (Ed.), Applied Plant Virology (pp. 763–775).
Li, W., Q. Song, R.H. Brlansky, and J.S. Hartung. 2007. Genetic diversity of citrus bacterial canker pathogens preserved in herbarium specimens. Proceedings of the National Academy of Sciences, 104(47): 18427–18432. https://doi.org/10.1073/pnas.0705590104
Luthra, J.C. and A. Sattar. 1942. Citrus canker and its control in Punjab. Punjab Fruit Journal, 6(1): 179–182
Marin, V.R., C.F.C. Zamuner, G.B. Hypolito, J.H. Ferrarezi, N. Alleoni, M.N. Caccalano, Ferreira, and D.C. Sass. 2024. Antibacterial activity of Cymbopogon species essential oils against Xanthomonas citri and their use in post-harvest treatment for citrus canker management. Letters in Applied Microbiology, 77(5) https://doi.org/10.1093/lambio/ovae041
Matrose, N.A., K. Obikeze, Z.A. Belay, and O.J. Caleb. 2021. Plant extracts and other natural compounds as alternatives for post-harvest management of fruit fungal pathogens: A review. Food Bioscience, 41: Article e100840. https://doi.org/10.1016/j.fbio.2020.100840
Mazhar, H. R., M. Atiq, N. A. Rajput, S. Ali, M. Usman, U. Ahmad, A. Nawaz, M. Faizan Ullah and S. Iqbal. 2021. Determination of Antibacterial Activity of Phytochemicals towards Xanthomonas citri Pv. citri Causing Citrus Canker. Agricultural Sciences Journal, 3(2): 1-12.
Moreira, L.M., N.F. Almeida, N. Potnis, L.A. Digiampietri, S.S. Adi, J.C. Bortolossi, A.C. Da Silva, A.M. Da Silva, F.E. De Moraes, J.C. De Oliveira, R.F. De Souza, A.P. Facincani, A.L. Ferraz, M.I. Ferro, L.R. Furlan, D.F. Gimenez, J.B. Jones, E.W. Kitajima, M.L. Laia, . . . J.C. Setubal. 2010. Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp. aurantifolii. BMC Genomics, 11(1): Article e238. https://doi.org/10.1186/1471-2164-11-238
Mubeen, M., A. Ali, Y. Iftikhar, M. Shahbaz, M.I. Ullah, Md.A. Ali, N. Fatima, J.S.S. Seelan, Y. S. Tan and U.B. Algopishi. 2024a. Innovative strategies for characterizing and managing huanglongbing in citrus. World Journal of Microbiology and Biotechnology. 40:342 https://doi.org/10.1007/s11274-024-04135-3
Mubeen, M., F. Bakhtawar, Y. Iftikhar, Q. Shakeel, A. Sajid, R. Iqbal, R.M. Aljowaie and T. Chaudhary. 2024b. Biological and molecular characterization of citrus bent leaf viroid. Heliyon. 10(7): Article e28209. https://doi.org/10.1016/j.heliyon.2024.e28209
Mubeen, M., H. M. Arshad, Y. Iftikhar, I. Bilqees, S. Arooj and H. M. Saeed. 2015a. In vitro efficacy of antibiotics against Xanthomonas axonopodis pv. citri through inhabitation zone techniques. International Journal of Agricultural and Applied Sciences. 7(1): 67–71
Mubeen, M., H. M. Arshad, Y. Iftikhar, M. U. Ullah and I. Bilqees. 2015b. Bio-chemical characterization of Xanthomonas axonopodis pv. citri: A gram-negative bacterium causing citrus canker. International Journal of Science and Nature, 6(2): 151–154
Nagy, J.K., A.M. Móricz, A. Böszörményi, A. Ambrus, and I. Schwarczinger. 2023. Antibacterial effect of essential oils and their components against Xanthomonas arboricola pv. pruni revealed by microdilution and direct bioautographic assays. Frontiers in Cellular and Infection Microbiology, 13https://doi.org/10.3389/fcimb.2023.1204027
Naqvi, S. a. H., J. Wang, M.T. Malik, U. Umar, N. Ateeq-Ur-Rehman, A. Hasnain, M.A. Sohail, M.T. Shakeel, M. Nauman, N. Hafeez-Ur-Rehman, M.Z. Hassan, M. Fatima, and R. Datta. 2022. Citrus canker—distribution, taxonomy, epidemiology, disease cycle, pathogen biology, detection, and management: A critical review and future research agenda. Agronomy, 12(5): Article e1075. https://doi.org/10.3390/agronomy12051075
Nauman, M., S. Mushtaq, M.F. Khan, A. Ali, S.A.H. Naqvi, Z. Haq and U.U.D. Umar. 2023. Morphological, biochemical, and molecular characterization of Xanthomonas citri subsp. citri, cause of citrus canker disease in Pakistan. Pakistan Journal of Botany, 55(6): 2409–2421
Nawaz, R., N.A. Abbasi, I.A. Hafiz, M.F. Khan, and A. Khalid. 2021. Environmental variables influence the developmental stages of the citrus leafminer, infestation level and mined leaves physiological response of Kinnow mandarin. Scientific Reports, 11(1): Article e7720. https://doi.org/10.1038/s41598-021-87160-8
Naz, M., D. Zhang, K. Liao, X. Chen, N. Ahmed, D. Wang, J. Zhou, and Z. Chen. 2024. The past, present, and future of plant activators targeting the salicylic acid signaling pathway. Genes, 15(9): Article e1237. https://doi.org/10.3390/genes15091237
Okoh, E.B., M. Payne, R. Lan, M. Riegler, T.A. Chapman and D.R. Bogema. 2024. A multilocus sequence typing scheme for rapid identification of Xanthomonas citri based on whole-genome sequencing data. Phytopathology, 114(7): 1480–1489 https://doi.org/10.1094/PHYTO-12-23-0490-R
Pathak, R., S.K. Singh, A. Tak, and P. Gehlot. 2018. Impact of climate change on host, pathogen and plant disease adaptation regime: a review. Biosciences Biotechnology Research Asia, 15(3): 529–540
Riasat, A., M.U. Ghazanfar and W. Raza. 2020. Interaction of citrus canker with Phyllocnistis citrella stainton. Pakistan Journal of Phytopathology, 32(1): 01-07.
Riseh, R.S., and M.G. Vazvani. 2024. Unveiling Methods to Stimulate Plant Resistance against Pathogens. Frontiers in Bioscience-Landmark, 29(5): Article e188. https://doi.org/10.31083/j.fbl2905188
Sadgrove, N.J., G.F. Padilla-González and M. Phumthum. 2022. Fundamental chemistry of essential oils and volatile organic compounds, methods of analysis and authentication. Plants, 11(6)https://doi.org/10.3390/plants11060789
Sebghatollahi, Z., M. Ghanadian, P. Agarwal, H.S. Ghaheh, N. Mahato, R. Yogesh, and S.H. Hejazi. 2022. Citrus flavonoids: Biological activities, implementation in skin health, and topical applications: A review. ACS Food Science & Technology, 2(9): 1417–1432
Shahbaz, E., M. Ali, M. Shafiq, M. Atiq, M. Hussain, R.M. Balal, A. Sarkhosh, F. Alferez, S. Sadiq, and M.A. Shahid. 2022. Citrus canker Pathogen, its mechanism of infection, eradication, and impacts. Plants, 12(1)https://doi.org/10.3390/plants12010123
Silva, A., C. Fernandes, D.D. Santos, M. Mazza, J. Silva, L. Magalhães, R. Pires, M. Miranda, and A. Crotti. 2024. Chemical composition, antileishmanial, and antifungal activities of essential oils from Cinnamomum cassia bark, Schinus molle dried leaves and their blends. ChemRxiv. https://doi.org/10.26434/chemrxiv-2024-xv7db
Sun, X., R.E. Stall, J.B. Jones, J. Cubero, T.R. Gottwald, J.H. Graham, W.N. Dixon, T.S. Schubert, P.H. Chaloux, V.K. Stromberg, G.H. Lacy, and B.D. Sutton. 2004. Detection and characterization of a new strain of citrus canker bacteria from Key/Mexican lime and alemow in South Florida. Plant Disease, 88(11): 1179–1188 https://doi.org/10.1094/PDIS.2004.88.11.1179
Tennant, P.F., D. Robinson, L. Fisher, S.M. Bennett, D. Hutton, P. Coates-Beckford, and W. McLaughlin. 2009. Diseases and pests of citrus (Citrus spp.). Tree and Forestry Science and Biotechnology, 3(2): 81–107
Testai, L., and V. Calderone. 2017. Nutraceutical value of citrus flavanones and their implications in cardiovascular disease. Nutrients, 9(5).https://doi.org/10.3390/nu9050502
Trade Development Authority of Pakistan (TDAP). (2023). Report on citrus production in Pakistan. TDAP Agricultural Reports.
Ullah, M.I., M. Riaz, M. Arshad, A.H. Khan, M. Afzal, S. Khalid, N. Mehmood, S. Ali, A.M. Khan, S.M.A. Zahid, and M. Riaz. 2019. Application of organic fertilizers affects the citrus leafminer, Phyllocnistis citrella (Lepidoptera: Gracillariidae) infestation, and citrus canker disease in nursery plantations. International Journal of Insect Science, 11: 1–12. https://doi.org/10.1177/1179543319858634
Vauterin, L., and P. Vauterin. 1995. Reclassification of Xanthomonas species based on DNA-DNA hybridization data. International Journal of Systematic and Evolutionary Microbiology, 45(3): 572–580
Villalta, O.N., M. Mebalds, and J. Edwards. 2018. Biosecurity tool-set: Optimising quarantine zones for eradication through consideration of Australia’s success with plant pathogen eradications in horticulture. Australasian Plant Pathology, 47: 135–154
Xiao, M., Y. Dong and X. Zhao. 2021. The role of vector insects in the spread of Xanthomonas axonopodis pv. citri. Journal of Agricultural and Urban Entomology, 37(1): 45–58 https://doi.org/10.1080/10509585.2021.1898875
Yan, Q. and N. Wang. 2012. High-throughput screening and analysis of genes of Xanthomonas citri subsp. citri involved in citrus canker symptom development. Molecular Plant-Microbe Interactions, 25: 69–84. https://doi.org/10.1094/MPMI-05-11-0121
Yu, Y., H. Liu, H. Xia and Z. Chu. 2023. Double-or triple-tiered protection: Prospects for the sustainable application of copper-based antimicrobial compounds for another fourteen decades. International Journal of Molecular Sciences, 24(13) https://doi.org/10.3390/ijms241310893
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