Resistance of Wheat Germplasm Against Spot Blotch and in Vitro Management Through Different Plant Extracts
DOI:
https://doi.org/10.33866/phytopathol.036.02.1221Keywords:
Spot blotch, Bipolaris sorokiniana, Wheat, in vitro Introduction,Abstract
Wheat serves as Pakistan's main source of dietary energy and has the title of being the country's most important grain crop. Spot blotch, a fungal disease caused by Bipolaris sorokiniana, is significantly impacting wheat yield s in the wake of rising temperature coupled with humid conditions during the crop season in Pakistan. This study investigated wheat germplasm resistance against the disease in NARC field conditions and in vitro botanical control measures. Out of 100 wheat lines, none was found to be highly resistant, 6 were found resistant and 5 showed moderate resistance. These six promising lines viz., TRB-95-KAC, TRB-62-127, BARS-KT-DNL-95, BARS-KT-DNL-102, BARS-KT-DNL-103, BARS-KT-DNL-105 are recommended for breeding program. Further research is needed to confirm these findings at other field locations. Pertaining to in vitro pathogen management, growth inhibition was found source and concentration dependent and had a positive correlation with the increase in concentrations within the same source/plant. Amongst three plant extracts used at various concentrations, highest antifungal activity against the pathogen in terms of growth inhibition was found @ 10% of garlic and Alstonia in PDA after 7 days’ incubation as compared to eucalyptus at the same concentration.References
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