Investigation of Citrus Melanose Disease in Silanwali, Sargodha (Pakistan)

Authors

  • Muhammad U. Hassan
  • Muhammad A. Zeeshan Assistant Professor Department of Plant Pathology, College of Agriculture, University of Sargodha, Sargodha
  • Yasir Iftikhar
  • Muhammad U. Ghani
  • Muhammad A. Shabbir
  • Sonum Bashir
  • Suleman Saleem
  • Komal Ambreen

DOI:

https://doi.org/10.33866/phytopathol.037.01.1261

Keywords:

Citrus melanose, Diaporthe citri, citrus disease, orchard management, irrigation, pruning, intercropping

Abstract

Citrus melanose deteriorates the marketable quality of citrus fruits; impacting the country’s economy to a considerable level. Sargodha is the citrus hub of the country where growers suffer from huge economic losses incited by citrus melanose. Assessment of factors affecting the citrus mealnose development in Silanwali would be effective to devise durable management practices for sustaining citrus industry in the region. The main objective of the survey is to explore the health status of citrus orchards regarding melanose disease. This study provides an area-specific survey for factor affecting mealnose and probable management tactics. A comprehensive survey was completed in Silanwali, Sargodha, to inspect citrus melanose regarding age of the orchard, type of irrigation (canal/tube well), intercropping, pruning practices, and weed infestation. The survey was based upon symptomology followed by laboratory analysis and farmer’s interviews regarding history and management options being practiced. The diseased samples were processed for fungal isolation and microscopic identification for confirmation before final data recording. The results indicated that the older orchards (25-35 years old) had a high disease incidence (30.1%), while in middle-aged (15-25 years old) and young orchards (5-15years old), about 20% and 15.4%, respectively. Tube well irrigation system data showed a 3.3% higher disease incidence over canal irrigation. A 1.6% rise in disease incidence was observed due to intercropping, weeds provide around 3.4% more incidence than incidence rate of intercropping does while pruning reduced up to 8% of the incidence of disease. PCA elucidated that weed, tube well irrigation, and intercropping was positively associated with diseases incidence while pruning and canal irrigation were negatively related, showing significant effects in reducing the diseases. The findings highlight the specific management options for mitigation of melanose. It could be concluded that sanitation, pruning and irrigation management are alternate options apart from fungicidal application to control melanose.

Author Biography

Muhammad A. Zeeshan, Assistant Professor Department of Plant Pathology, College of Agriculture, University of Sargodha, Sargodha

Assistant professorDepartment of Plant Pathology,University College of Agriculture,University of Sargdoha, Sargodha.

References

Ateeq, M., M. Mubeen, S. Bashir, R.T. Bajwa, H.M. Imran, A.A. Arshad and M.D.C.Z. Romano. 2023. Etiology and management of citrus Melanose disease in Pakistan: A review. Phytopathogenomics and Disease Control, 2: 29-36.

Atiq, M., L. Malik, N.A. Rajput, M. Shabbir, M. Ashiq, M.J. Matloob, A. Waseem, A. Jabbar, A. Ullah, R. Zawar and A. Majeed. 2024. Evaluating the efficacy of plant-mediated copper-silver nanoparticles for controlling cercospora leaf spot in mung beans. Plant Protection, 08 (04): 661-670.

Anwar, U., M. Mubeen, Y. Iftikhar, M.A. Zeshan, Q. Shakeel, A. Sajid, M. Umer and A. Abbas. 2021. Efficacy of different fungicides against citrus melanose disease in Sargodha, Pakistan. Pakistan Journal of Phytopathology, 33(1).

Bhatti, A.M., H.M. Usman, A. Iffat, M. Tatar, M.M. Karim, M.I. Zafar and T. Shafique. 2024. Deciphering the current scenario and prospective outlook of citrus gummosis in Pakistan. Düzce Üniversitesi Ziraat Fakültesi Dergisi, 2(1): 46-59.

Chaisiri, C., X. Liu, Y. Lin and C. Luo. 2022. Diaporthe citri: a fungal pathogen causing melanose disease. Plants, 11(12): 1600.

Feng, H.U.A.N.G., P.E.N.G. Aitian, S.O.N.G. Xiaobing, C.U.I. Yiping, L.I.N.G. Jinfeng, C.H.E.N. Xia and L.I. Hongye. 2024. Research progress of citrus melanose. Guangdong Agricultural Sciences, 51(6): 22-33.

Ferro, L.O., J.D.P. Bezerra, T.M. Da Silva, C.S. De Oliveira, S. dos S Nascimento, L.M. Paiva and C.M. Souza-Motta. 2024. Endophytic Diaporthe species from Brazil. Fungal Systematics and Evolution, 14(1): 251-270.

Goyal, A., and K. Lakhwani. 2025. An extensive survey on citrus plant disease detection using image processing and deep learning. Artificial Intelligence in Medicine and Healthcare, 95-119.

Kalal, P., D.V.V. Manisha, A.K. Mahawer, A.J. Sachin and K.N. Vs. 2023. Impact of salinity stress on citrus production and its alleviation strategies. The Pharma Innovation Journal, 12(6): 3620-3626.

Lai, D., D. Wang, R. Lai, X. Shao, J. Qin, Q. Zhuang and W. Xiao. 2024. Diaporthe arecae is a new pathogen causing Chinese olive (Canarium album) dieback in China. Physiological and Molecular Plant Pathology, 133: 102340.

Liu, T., Z. Zhou, C. Luo, H. Luo, J. Tang, X. Shi and T. Yi. 2024. Elucidation of mechanisms underlying active oxygen burst in Citrus sinensis after Diaporthe citri infection using transcriptome analysis. Frontiers in Microbiology, 15: 1425441.

Matias, P., I. Barrote, G. Azinheira, A. Continella and A. Duarte. 2023. Citrus pruning in the Mediterranean climate: A review. Plants, 12(19): 3360.

Martino, I., G. Lione, M. Garbelotto, P. Gonthier and V. Guarnaccia. 2024. Modeling the effect of temperature on the severity of Blueberry Stem Blight and Dieback with a focus on Neofusicoccum parvum and Cultivar susceptibility. Horticulturae, 10(4): 363.

Mir, M.S., A. Saxena, R.H. Kanth, W. Raja, K.A. Dar, S.S. Mahdi and S.A. Mir. 2022. Role of intercropping in sustainable insect-pest management: A review. International Journal of Environment and Climate Change, 12(11): 3390-3404.

Moyo, P., R.O. Fialho, G.J. Silva Junior and P. Fourie. 2025. Citrus Black Spot symptoms on fruit exposed to Phyllosticta citricarpa infections at different developmental stages. Plant‐Environment Interactions, 6(1): e70031.

Pu, Z., F. Huang, L. Zhu, D. Du, X. Hu, L. Lu and Z. Huang. 2024. A novel TaqMan qPCR system for the detection of Diaporthe citri, the causative agent of citrus melanose. Biotechnology & Biotechnological Equipment, 38(1): 2338437.

Rehman, F., M. Kalsoom, A. Sultan, S. Junaid, H. Akram, M. Tariq, T. Shafique and M. Zafar. 2020. Citrus melanose and quality degradation of fruit by this disease: A Review. Open Access Journal of Biogeneric Science and Research, 3(5):1-4.

Rymbai, H. 2024. Organic Cultivation of citrus (Citrus spp.). In: Organic culture of Tropical and Subtropical fruit plants (Eds. Ghosh, S.N., Krishna Hare, Rymbai, H). Publisher Gyanavi Publishers & Distributors. ISBN 978-81-960111-5-4. pp 131-176. doi: 10.13140/RG.2.2.36585.51049.

Safdar, A., N. Javed, S.A. Khan, H.U. Khan, A. Rehman and I.U. Haq. 2010. Survey and investigation of different citrus growing areas for citrus sudden death syndrome. Pakistan Journal of Phytopathology, 22(2): 71-78.

Ur Rehman, K., M.W. Akram, N. Ishaq, N. Akram and S. Andleeb. 2024. Comparative assessment of fungicide treatments on Meyer lemon quality and productivity. International Journal of Environmental Science and Technology, 21(5): 4913-4926.

Usman, M., M. Atiq, N.A. Rajput, S.T Sahi, M. Shad, N. Lili, S. Iqbal, A.M Arif, U. Ahmad, K.S. Khan, M. Asif and F.U. Haider. 2024. Efficacy of green synthesized silver-based nanomaterials against early blight of tomato caused by Alternaria solani. Gesunde Pflanzen, 76(1): 105-115.

Usman, M., M. Atiq, N.A. Rajput, M. Sagheer, Y. Xia. 2025. Computational and experimental approaches to explore defense related enzymes conferring resistance in Fusarium infected chilli plants by regulating plant metabolism through nutritional products. PLoS One, 20(1):e0309738

Ziogas, V., G. Tanou, G. Morianou and N. Kourgialas. 2021. Drought and salinity in citriculture: Optimal practices to alleviate salinity and water stress. Agronomy, 11(7), 1283.

Zhou, Y., L. Yin, W. Han, C. Chaisiri, X. Liu, X. Yue and P. Li. 2025. Identification and characterization of Diaporthe citri as the causal agent of Melanose in Lemon in China. Plants, 14(12): 1771.

Zhou, Z., S. Lu, T. Liu, J. Liu, J. Deng, X. Lu and T. Yi. 2024. Biocontrol of citrus melanose Diaporthe citri by Bacillus subtilis M23. Biological Control, 197: 105608.

Published

2025-06-30

How to Cite

Hassan, M. U., Zeeshan, M. A., Iftikhar, Y., Ghani, M. U., Shabbir, M. A., Bashir, S., Saleem, S., & Ambreen, K. (2025). Investigation of Citrus Melanose Disease in Silanwali, Sargodha (Pakistan). Pakistan Journal of Phytopathology, 37(1), 73-79. https://doi.org/10.33866/phytopathol.037.01.1261

Issue

Section

Research Articles

Most read articles by the same author(s)

> >>