GANODERMA LUCIDUM: A CASE STUDY FOR LACCASE BIOSYNTHESIS

Authors

  • Zill-e-Huma Aftab Institute of Agricultural Sciences, University of the Punjab, Lahore
  • Shakil Ahmed Department of Botany, University of the Punjab, Lahore

Keywords:

Ganoderma lucidum, laccase, submerged fermentation, white rot fungus

Abstract

Laccase production by white rot fungus Ganoderma lucidum (Curtis) P. Karst. was investigated through submerged fermentation using effluents of paper and pulp industry as substrate. The effect of variation in culture and nutritional conditions was optimized for industrial scale production of enzyme. Among the optimized parameters incubation period 16 days, inoculums size 13 discs (5 mm), initial pH 5, incubation temperature 30 oC,  glucose 4.0 g 50 mL-1 and yeast extract 2 g 50 mL-1 were found the best for laccase synthesis (22.54 U mL-1). Optimum pH and temperature for enzyme activity were 3.0 and 65 oC. The laccsae activity was strongly inhibited by cobalt at 1 mM and 5 mM concentrations.

Published

2015-08-21

How to Cite

Aftab, Z.- e-H., & Ahmed, S. (2015). GANODERMA LUCIDUM: A CASE STUDY FOR LACCASE BIOSYNTHESIS. Pakistan Journal of Phytopathology, 27(1), 95-103. Retrieved from http://www.pjp.pakps.com/index.php/PJP/article/view/174

Issue

Vol. 27 No. 1 (2015): Pak. J. Phytopath.

Section

Research Articles

Submission of a manuscript infers that the work described has not been published before (except in the form of an abstract or as part of a published lecture, or thesis). It is also not under consideration for publication elsewhere.

All works published by Pakistan Journal of Phytopathology is freely available to copy, distribute, transmit and adapt the work provided the original work and source is appropriately cited.