Dalila Amokrane, Ahmed Mohammedi, Meryem Belabbes, Hadjer Tegger, Abdelaziz Merouane


This study aims to determine the composition of the essential oil of Pistacia lentiscus leaves by gas chromatography-mass spectrometry (GC/MS) and to test its insecticidal activity against the larvae of Aphis spiraecola, which represents a serious threat to citrus production and cause most economic loss for the citrus culture. The essential oil of lentisk leaves was isolated by hydrodistillation using a Clevenger-type, the chemical composition was determined by GC/MS. The insecticidal activity of essential oil was determined by using the contact method against A. spiraecola larvae. A total of 74 compounds were identified, corresponding to chromatographic peaks representing 89.6% of the total area of all peaks. The most abundant compounds were monoterpene hydrocarbons (54.2%) with 8.8% p-cymene and 7.2% α-pinene. The insecticidal assay revealed an interesting insecticidal activity against the larvae of A. spiraecola with an LD50 of 0.2 µL. This study introduces and supports the use of the essential oil of P. lentiscus as a biopesticide and open new ways for its future exploitation in phytosanitary industries.


Citrus culture, essential oil; biopesticide; Aphis spiraecola; insecticidal activity

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Abbott, W. S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265-267.

Abdelmaksoud, N. M., A. M. El-Bakry, E. A. Sammour and N. F. Abdel-Aziz. 2023. Comparative toxicity of essential oils, their emulsifiable concentrates and nanoemulsion formulations against the bean aphid, Aphis fabae. Archives of Phytopathology and Plant Protection, 56(3): 187-208.

Aissi, O., M. Boussaid and C. Messaoud. 2016. Essential oil composition in natural populations of Pistacia lentiscus L. from Tunisia. Effect of ecological factor and incidence on antioxidant and anti-acetyl cholinesterase activities. Industrial Crops and Products, 91: 56–65.

Amara, N., A. Benrima, C. Anba and H. Belkhir. 2019. Activité antimicrobienne de l’huile essentielle des fruits du pistachier lentisque (Pistacia lentiscus L.). Revue Agrobiologia, 9(2): 1669-1676.

Bakkali, F., S. Averbeck, D. Averbeck and M. Idaomar. 2008. Biological effects of essential oils-A review. Food and Chemical Toxicology, 46: 446-475.

Batsc, D. 2011. L’impact des pesticides sur la santé humaine. Thèse de diplôme d’état en pharmacie. Université Henri Poincarde, Nancy, pp.185.

Behi, F., O. Bachrouch and S. Boukhris-Bouhachem. 2019. Insecticidal Activities of Mentha pulegium L., and Pistacia lentiscus L., Essential Oils against Two Citrus Aphids Aphis spiraecola Patch and Aphis gossypii Glover. Journal of Essential Oil Bearing Plants, 22(2): 516-525.

Ben, D. F., N. Hayder, L. Chekir-Ghedira, M. Hammami, K. Ghedira, A. M. Mariotte and M. G. Dijoux-Franca. 2005. New study of the essential oil from leaves of Pistacia lentiscus L. (Anacardiaceae) from Tunisia. Flavour and Fragrance, 20: 410–414.

Bernays, E. A. and R. F. Chapman. 1998. Chemicals in Plants. In: E. A. Bernays, R. E. Chapman (eds). Host-Plant Selection by Phytophagous Insects. Contemporary Topics in Entomology, volume2. Springer, Boston, MA, USA.

Blackman, R. L. and V. F. Eastop. 2006. Aphids on the world’s herbaceous plants and shrubs. Ed. John Wiley and Sons Incorporation, England.

Bouyahyaoui, A., F. Bahri, A. Romane, M. Hoferl, J. Wanner, E. Schmidt and L. Jirovetz. 2016. Antimicrobial Activity and Chemical Analysis of the Essential Oil of Algerian Juniperus phoenicea. Natural Product Communications,11(4): 519-522.

Burger, P., H. Plainfossé, X. Brochet, F. Chemat and X. Fernandez. 2019. Extraction of natural fragrance ingredients: history overview and future trends. Chemistry & Biodiversity, 16(10): e1900424.

CAPE (Canadian Association of Physicians for the Environment). 2009. Position Statement on Synthetic Pesticides. https://cape.ca

Clevenger, J. F. 1928. Appareil pour la détermination de l'huile volatile, Description du nouveau type. Le Journal de l'Association Pharmaceutique Américaine, 17: 345-349.

Colignon, P., E. Haubruge, C. Gaspar and F. Francis. 2003. Effets de la réduction des doses de formulations d’insecticides et de fongicides sur l’insecte auxiliaire non ciblé Episyrphus balteatus [Diptera: Syrphidae] . Phytoprotection, 84(3) :141-148.

De Menezes, M. 1970. Reproducao sexuada de Aphis spiraecola Patch no estado de Sao Paulo. Biológico, 36: 53-57.

El Bishbishy, M.H., H.A. Gad and N.M. Aborehab. 2020. Chemometric discrimination of three Pistacia species via their metabolic profiling and their possible in vitro effects on memory functions. Journal of Pharmaceutical and Biomedical Analysis, 177: 112840.

Gardeli, C., V. Papageorgiou, A. Mallouchos, K. Theodosis and M. Komaitis. 2008. Essential oil composition of Pistacia lentiscus L. and Myrtus communis L. Evaluation of antioxidant capacity of methanolic extract. Food Chemistry, 107: 1120–1130.

Govindarajan, M., M. Rajeswary and G. Benelli. 2016. delta-Cadinene, calarene and delta-4-carene from Kadsura heteroclita essential oil as novel larvicides against malaria, dengue and filariasis mosquitoes. Combinatorial Chemistry & High Throughput Screening, 19(7):565-571.

Hodjat, S. H. and V. F. Eastop. 1983. Aphis citricola van der Goot, a new aphid pest of citrus in Iran. Entomologie et Phytopathologie Appliquées, 50(1/2): 57-66.

Hu, H-L., D. Zhou, J-W. Wang, C. Wu, H-J. Li, J. Zhong, Z. Xiang and C. Sun. 2022. Chemical Composition of Citronella (Cymbopogon winterianus) Leaves Essential Oil and Gastric Toxicity of Its Major Components to Drosophila melanogaster Larvae. Journal of Essential Oil Bearing Plants, 25(2): 315-325.

Hullé, M., E. Turpeau and B. Chaubet. 2012. Encyclop’Aphid, tout savoir sur les pucerons. INRA Magazine, Université de Renne 1, Renne, France.

Ikbal, C. and R. Pavela. 2019. Essential oils as active ingredients of botanical insecticides against aphids. Journal of Pest Science, 92: 971-986.

Kavallieratos, N. G., M. C. Boukouvala, C. T. Ntalaka, A. Skourti, E. P. Nika, F. Maggi, E. Spinozzi, E. Mazzara, R. Petrelli, G. Lupidi, C. Giordani and G. Benelli. 2021. Efficacy of 12 commercial essential oils as wheat protectants against stored-product beetles, and their acetylcholinesterase inhibitory activity. Entomologia Generalis, 41: 385-414.

Khanikor, B., P. Parida, R.N.S. Yadav and D. Bora. 2013. Comparative mode of action of some terpene compounds against octopamine receptor and acetyl cholinesterase of mosquito and human system by the help of homology modeling and docking studies. Journal of Applied Pharmaceutical Science, 3(2): 6-1.

Komazaki, S. 1991. Studies on the biology of the spirea aphid, Aphis spiraecola Patch, with special reference to biotypic differences. Bulletin of the Fruit Tree Research Station, Extra No. 2: 60.

Lucia, A., A.P. Gonzalez, S. Licastro and H. Masuh. 2007. Larvicidal effect of Eucalyptus grandis essential oil and turpentine and their major components on Aedes Aegypti larvae. Journal of the American Mosquito Control Association, 23(3): 299-303.

Michaelakis, A., A.T. Strongilos, E.A. Bouzas, G. Koliopoulos and A. Elias. 2009. Larvicidal activity of naturally occurring naphthoquinones and derivatives against the West Nile virus vector Culex pipiens. Parasitology Research, 104: 657–662.

Michaelakis, A., D. Papachristos, A. Kimbaris, G. Koliopoulos, A. Giatropoulos, G. Moscho and M. Polissiou. 2009. Citrus essential oils and four enantiomeric pinenes against Culex pipiens (Diptera: Culicidae). Parasitology Research, 105: 769–773.

Nejad, F.Y., R. Rajabi and N. Palvaneh. 2013. A review on evaluation of plant essential oils against pests in Iran. Journal of Persian Gulf Crop Protection, 2 (4): 74-97.

Papachristos, D.P. and D.C. Stamopoulos. 2002. Repellent, toxic and reproduction inhibitory effects of essential oil vapours on Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae). Journal of Stored Products Research, 38: 117–128.

Pavela, R. and G. Benelli, G. 2016. Essential oils as ecofriendly biopesticides? Challenges and constraints. Trends in Plant Science, 21: 1000-1007.

Pfeiffer, D.G. 1991. Biology and management of aphids on apple, In: K. Williams, (Ed.), New Directions in Tree Fruit Pest Management. Good Fruit Grower Yakima, Washington, United States of America.

Rajendran, S. and V. Sriranjini. 2008. Plant products as fumigants for stored-product insect control. Journal of Stored Products Research, 44: 126-135.

Rauf, A., S. Patel, G.B. Uddin, S. Siddiqui, B. Ahmad, N. Muhammad, Y.N. Mabkhot and T. Ben Hadda. 2017. Phytochemical, ethnomedicinal uses and pharmacological profile of genus Pistacia. Biomed Pharmacotherapy, 86: 393-404.

Sehaki, C., N. Jullian, E. Choque, R. Dauwe and J.X. Fontaine. 2022. Profiling of Essential Oils from the Leaves of Pistacia lentiscus Collected in the Algerian Region of Tizi-Ouzou: Evidence of Chemical Variations Associated with Climatic Contrasts between Littoral and Mountain Samples. Molecule, 27: 41-48.

Sener, O., M. Arslan, N. Demirel and I. Uremis. 2009. Insecticidal effects of some essential oils against the confused flour beetle (Tribolium confusum du Val) (Col.: Tenebrinoidea) in stored wheat. Asian Journal of Chemistry, 21(5):3995-4000.

Stefanazzi, N., T. Stadler and A. Ferrero. 2011. Composition and toxic, repellent and feeding deterrent activity of essential oils against the stored-grain pests Tribolium castaneum (Coleoptera: Tenebrionidae) and Sitophilus oryzae (Coleoptera: Curculionidae). Pest Management Science, 67: 639–646.

Tabti, L., M. Dib, B. Tabti, J. Costa and A. Muselli. 2020. Insecticidal Activity of Essential Oils of Pistacia atlantica Desf. and Pistacia lentiscus L. Against Tribolium confusum Dul. Journal of Applied Biotechnology Reports, 7(2): 111-115.

Vidrich, V., P. Fusi, A. Graziano, E. Silvestrini, M. Michelozzi and F. Marco. 2004. Chemical composition of the essential oil of Pistacia Lentiscus L. Journal of Essential Oil Research, 16: 223-226.

DOI: https://doi.org/10.33866/phytopathol.035.02.0976


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