BIO-CONTROL EFFECT OF ERUCA SATIVA MILL. OIL AGAINST THE HAZARDOUS FOOD BORNE PATHOGENS
Keywords:
E. sativa oil, antimicrobial activity, phytopathogens.Abstract
ABSTRACTAntimicrobial activity of Eruca sativa Mill. oil was evaluated against phytopathogenic bacterial species (Xenorhabdus luminescens, Acinetobacter sp., Bordetella pertussis, Ensifer adhaerens, Pseudomonas syringae, Acidovorax temperans, Xanthomonas axonopodis) and fungal species (Alternaria alternata, Dreschlera halodes, Aspergillus nidulans, Acremoniun kiliense, Fusarium oxysporum, Curvularia clavata, Rhizopus oryzae). Antifungal activity was determined on MEA while antibacterial activity on NA media plates to measure the effects of oil. The antimicrobial activity was tested by well diffusion method in vitro. E. sativa oil was found to be highly active against all fungal isolates tested as compared to bacterial isolates. Results showed evidence of high antibacterial activity against X. luminescens with inhibition zone of 3.1 cm. E. adhaerens and A. temperans exhibited least resistance against oil with 1.4 cm and 1.7 cm zone of inhibition respectively. The oil showed high antifungal activity in the range of 6.0-6.8cm inhibition zone against D. halodes, C. clavata, R. oryzae and A. nidulans whereas least active against F. oxysporum with 1.1cmzone of inhibition. The antimicrobial components from this oil can be used as an alternative to develop novel pesticides by replacing some chemical commercial antifungal and antibacterial for the plant diseases.Published
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