CHARACTERIZATION OF GROWTH PROMOTING RHIZOBACTERIA OF LEGUMINOUS PLANTS

Abstract

The present investigation deals with the morphological and biochemical identification of rhizobacteria having plant growth promoting characteristics, with a focus on the family Papilionaceae, whose representatives are widespread in nature as leguminous crops. Eleven bacterial strains were isolated from the rhizoplane of pea (Pisum sativum L.), chickpeas (Cicer arietinumL.) and clover (Trifolium pretense L.). The media used to isolate the rhizosphere bacteria were Luria Bertani (LB) agar and Nutrient agar (NA). Rhizosphere bacteria were identifiedbased on different characteristic i.e. phenotypic and biochemical characters. The isolated strains were characterized and assigned to various genera and species, such as Enterobacter aerogenes, Aureobacterium flavescens, Pseudomonas fluorescens, Kurthiasp., Acidovorax facilis, Bordetella pertussis, Corynebacterium sp., Bacillus sp., Curtobacterium albidum and Microbacterium lacticum. All identified species are given FCBP accession numbers and were stored for further use.