COMBRETUM INDICUM – A NEW HOST RECORD OF ALTERNARIA BRASSICAE LEAF SPOT DISEASE FROM PAKISTAN

Authors

  • Naureen Akhtar Institute of Agricultural Sciences, University of the Punjab Lahore Pakistan
  • Rahila Hafeez Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.
  • Waheed Anwar Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan.

DOI:

https://doi.org/10.33866/phytopathol.029.01.0348

Keywords:

Alternaria brassicae, Combretum indicum, leaf spot, pathogenicity

Abstract

Combretum indicum leaf spot disease was observed during a survey of horticultural plants in Lahore. Isolation of causal organism was carried out from the disease leaves. The pathogen, Alternaria brassicae, was first identified considering morphological characters. The morphology based identification was confirmed by nucleotide sequence analysis of ITS region of rDNA and phylogentic analysis of this fungus with closely related other fungal species. Pathogenicity of A. brassicae was verified following the Koch’s pathogenicity postulates. This report represents the first record of leaf spot of Combretum indicum by Alternaria brassicae from Pakistan.

References

Akhtar N., Shoaib A., Munir S., Ali A. and Khurshid S., 2014. Isolation, identification and enzyme production profile of Aspergillus niger. Pakistan J. Animal and Pl. Sci., 24(5): 1438-1443.

Czajka A., Czubatka A., Sobolewski J. and Robak J., 2015. First report of Alternaria leaf spot caused by Alternaria alternata on spinach in Poland. APS Plant Disease, 99: (5): 729-729.

Felsenstein J., 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39: 783-791.

Javaid A., Akhtar N., Khan A., Shoaib A. and Hafeez R., 2016. New host record of Alternaria brassicicola infecting Triangle palm (Dypsis decaryi) in Pakistan. Pakistan J Animal and Plant Sci., 26(6): 1894-1898.

Kirk P. M., Cannon P. F., Minter D. W. and Stalpers J. A., 2008. Dictionary of the Fungi. 10th ed., Wallingford, CABI. pp 22.

Kumar D., Maurya N., Bharti Y. K., Kumar A., Kumar K., Srivastava K., Chand G., Kushwaha C., Singh S. K., Mishra R. K. and Kumar A., 2014. Alternaria blight of oilseed brassicas: A comprehensive review. African J. Microbiol. Res., 8: 2816-2829.

Rout P. K., Naik S. N. and Rao Y. R., 2008. Subcritical CO2 extraction of floral fragrance from Quisqualis indica. The J. of Supercritical Fluids, 45(2): 200-205.

Simmons E.G., 2007. Alternaria: An Identification Manual. CBS Fungal Biodiversity Center Utrecht, Netherlands.

Tamura K. and Nei M., 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10: 512-526.

Tamura K., Stecher G., Peterson D., Filipski A. and Kumar S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30: 2725-2729.

White T.J., Bruns T. and Lee S., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Taylor J., Innis A., Gelfand D.H., Sninsky J.J. (eds). PCR Protocols, pp. 315-322. Academic Press, San Diego, CA, USA.

Published

2017-07-12

How to Cite

Akhtar, N., Hafeez, R., & Anwar, W. (2017). COMBRETUM INDICUM – A NEW HOST RECORD OF ALTERNARIA BRASSICAE LEAF SPOT DISEASE FROM PAKISTAN. Pakistan Journal of Phytopathology, 29(1), 171-173. https://doi.org/10.33866/phytopathol.029.01.0348

Issue

Vol. 29 No. 1 (2017): Pak. J. Phytopath.

Section

First Reports

Submission of a manuscript infers that the work described has not been published before (except in the form of an abstract or as part of a published lecture, or thesis). It is also not under consideration for publication elsewhere.

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