EVALUATION OF MYCOCHEMICAL PROFILE OF ALTERNARIA JAPONICA THROUGH GC-MS ANALYSIS

Authors

  • Arshad Javaid Institute of Agricultural Sciences, University of the Punjab Lahore
  • Iqra H. Khan Institute of Agricultural Sciences, University of the Punjab Lahore, Pakistan.
  • Khajista Jabeen Department of Botany, Lahore College for Women University, Lahore, Pakistan
  • Uzma Bashir Institute of Agricultural Sciences, University of the Punjab Lahore, Pakistan

Keywords:

Alternaria japonica, antimicrobial, antioxidant, GC-MS analysis, mycochemicals

Abstract

The present study was carried out to find out mycochemical profile of culture filtrates of Alternaria japonica through GC-MS analysis. The fungus was incubated for 21 days using malt extract broth as growth medium. Culture filtrate was filtered and concentrated by evaporating water at 45 °C. Filtrates were partitioned using n-hexane followed by chloroform. Both the organic solvent fractions were analyzed through GC-MS. In n-hexane and chloroform fractions, 14 and 10 compounds were identified, respectively. Major compounds in n-hexane fraction were o-xylene (36.9%); ethyl benzene (19.3%), hexane, 2-4-dimethyl- (16.8%), 1-2 dimethylbenzene (7.56%), cyclohexane, 1-ethyl-1-methyl (3.36%), heptane, 2,5-dimethyl- (3.36%) and octane, 4-methyl- (2.52%). Likewise, predominant compounds in chloroform fraction were octane-4 methyl (24.62%), carbonic dihydrazine (16.41%), octane, 2,4,6-trimethyl (12.56%), hydrazine, (1,1-dimethylethyl) (8.75%), 16-hexadecanoyl hydrazide (7.66%) and stearic acid hydrazide (7.08%). Literature surveyed showed that many compounds identified in both the fractions possess antimicrobial, pharmacological, antioxidant, antidepressant, anticancer and anti-inflammatory properties.

Author Biographies

Arshad Javaid, Institute of Agricultural Sciences, University of the Punjab Lahore

Associate ProfessorInstitute of Agricultural Sciences, University of the Punjab Lahore, Pakistan.

Iqra H. Khan, Institute of Agricultural Sciences, University of the Punjab Lahore, Pakistan.

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Khajista Jabeen, Department of Botany, Lahore College for Women University, Lahore, Pakistan

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Uzma Bashir, Institute of Agricultural Sciences, University of the Punjab Lahore, Pakistan

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References

Akhtar, R. and A. Javaid. 2018. Biological Management of Basal Rot of Onion by Trichoderma harzianum and Withania somnifera. Planta Daninha. 36.

Al Ghafri, A., S.S.N. Maharachchikumbura, K.D. Hyde, N.A. Al-Saady and A.M. Al-Sadi. 2019. A new section and a new species of Alternaria encountered from Oman. Phytotaxa-Biotaxa. 405:279.

Al‐Lami, H., M. You and M. Barbetti. 2019. Incidence, pathogenicity and diversity of Alternaria spp. associated with alternaria leaf spot of canola (Brassica napus) in Australia. Plant Pathology. 68:492-503.

Chandra, S., Vandana and S. Kumar. 2015. Synthesis, spectroscopic, anticancer, antibacterial and antifungal studies of Ni(II) and Cu(II) complexes with hydrazine carboxamide, 2-[3-methyl-2-thienyl methylene]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 135:356-363.

Da Cruz Cabral, L., A. Rodríguez, J. Delgado and A. Patriarca. 2019. Understanding the effect of postharvest tomato temperatures on two toxigenic Alternaria spp. strains: growth, mycotoxins and cell‐wall integrity‐related gene expression. Journal of the Science of Food and Agriculture. 99:6689-6695.

Dang, H.X., B. Pryor, T. Peever and C.B. Lawrence. 2015. The Alternaria genomes database: a comprehensive resource for a fungal genus comprised of saprophytes, plant pathogens, and allergenic species. BMC Genomics. 16.

Firdouse, S., H.F. Begum and P. Alam. 2019. GC–MS Analysis of Bioactive Compounds Present in Marketed Herbal Formulation Pykure Capsule. Journal of Drug Delivery and Therapeutics. 9:73-104.

Gambacorta, L., N. El Darra, R. Fakhoury, A.F. Logrieco and M. Solfrizzo. 2019. Incidence and levels of Alternaria mycotoxins in spices and herbs produced worldwide and commercialized in Lebanon. Food Control. 106:106724.

Gelain, A., M. Mori, F. Meneghetti, F. Porta, L. Basile, G. Marverti, A. Asai, M. Hyeraci, A.N. García-Argáez, L.D. Via, S. Guccione and S. Villa. 2018. Exploring the Biological Activity of a Library of 1,2,5-Oxadiazole Derivatives Endowed With Antiproliferative Activity. Anticancer Research. 39:135-144.

Gilani, S.J., S.A. Khan, N. Siddiqui, S.P. Verma, P. Mullick and O. Alam. 2011. Synthesis and in vitro antimicrobial activity of novel N-(6-chlorobenzo [d] thiazol-2-yl) hydrazine carboxamide derivatives of benzothiazole class. Journal of enzyme inhibition and medicinal chemistry. 26:332-340.

Girija, S., V. Duraipandiyan, P.S. Kuppusamy, H. Gajendran and R. Rajagopal. 2014. Chromatographic Characterization and GC-MS Evaluation of the Bioactive Constituents with Antimicrobial Potential from the Pigmented Ink of Loligo duvauceli. International Scholarly Research Notices. 2014:1-7.

Harikrishnan, R., M.-C. Kim, J.-S. Kim, C. Balasundaram, S. Jawahar and M.-S. Heo. 2010. Identification and Antimicrobial Activity of combined extract from Azadirachta indica and Ocimum Sanctum.

Int, C. 2019. GC/MS characterization of volatile components of hydrocolloids from Irvingia gabonensis and Mucuna sloanei seeds.

Iqbal, S., M. Saleem, M.K. Azim, M. Taha, U. Salar, K.M. Khan, S. Perveen and M.I. Choudhary. 2017. Carbohydrazones as new class of carbonic anhydrase inhibitors: Synthesis, kinetics, and ligand docking studies. Bioorganic Chemistry. 72:89-101.

Jubie, S., P.N. Ramesh, P. Dhanabal, R. Kalirajan, N. Muruganantham and A. Shanish Antony. 2012. Synthesis, antidepressant and antimicrobial activities of some novel stearic acid analogues. European Journal of Medicinal Chemistry. 54:931-935.

Koçyiğit-Kaymakçıoğlu, B., E.E. Oruç-Emre, S. Ünsalan, N. Tabanca, S.I. Khan, D.E. Wedge, G. İşcan, F. Demirci and S. Rollas. 2012. Synthesis and biological activity of hydrazide–hydrazones and their corresponding 3-acetyl-2,5-disubstituted-2,3-dihydro-1,3,4-oxadiazoles. Medicinal Chemistry Research. 21:3499-3508.

Kumar, D., R. Narang, V. Judge, D. Kumar and B. Narasimhan. 2012. Antimicrobial evaluation of 4-methylsulfanyl benzylidene/3-hydroxy benzylidene hydrazides and QSAR studies. Medicinal Chemistry Research. 21:382-394.

Mokale, S.N., S.S. Shinde, R.D. Elgire, J.N. Sangshetti and D.B. Shinde. 2010. Synthesis and anti-inflammatory activity of some 3-(4,6-disubtituted-2-thioxo-1,2,3,4-tetrahydropyrimidin-5-yl) propanoic acid derivatives. Bioorganic & Medicinal Chemistry Letters. 20:4424-4426.

Patriarca, A., L. Da Cruz Cabral, M.A. Pavicich, K.F. Nielsen and B. Andersen. 2019. Secondary metabolite profiles of small-spored Alternaria support the new phylogenetic organization of the genus. International Journal of Food Microbiology. 291:135-143.

Peng, W., Z. Lin, J. Chang, F. Gu and X. Zhu. 2013. Biomedical molecular characteristics of YBSJ extractives from Illicium verum fruit. Biotechnology & Biotechnological Equipment. 27:4311-4316.

Peng, W., Z. Lin, J. Chang, F. Gu and X. Zhu. 2013. Biomedical Molecular Characteristics of YBSJ Extractives fromIllicium VerumFruit. Biotechnology & Biotechnological Equipment. 27:4311-4316.

Sabitha, G., A.S. Rao, A. Sandeep, B.M. Latha and D.V. Reddy. 2014. Total synthesis of (−)-synrotolide and the evaluation of its antiproliferative activity. Tetrahedron: Asymmetry. 25:856-859.

Siciliano, I., S. Franco Ortega, G. Gilardi, P. Bosio, A. Garibaldi and M.L. Gullino. 2018. Molecular phylogeny and characterization of secondary metabolite profile of plant pathogenic Alternaria species isolated from basil. Food Microbiology. 73:264-274.

Sivakumar, D., F. Van Deventer, L.A. Terry, G.A. Polenta and L. Korsten. 2011. Combination of 1-methylcyclopropene treatment and controlled atmosphere storage retains overall fruit quality and bioactive compounds in mango. Journal of the Science of Food and Agriculture. 92:821-830.

Sumathy, V.J.H. 2016. Comparative Analys. Int. J. Curnt. Tren. Pharm, Res. 4:338-344.

Tabarestani, M. S., K. Rahnama, M. Jahanshahi, S. Nasrollanejad and M. Fatemi. 2016. Identification of Volatile Organic Compounds from Trichoderma virens (6011) by GC-MS and Separation of a Bioactive Compound via Nanotechnology. International Journal of Engineering-Transactions A: Basics, 29: 1347-1353.

Ubaid, J.M., M.J. Kadhim and I.H. Hameed. 2016. Study of bioactive methanolic extract of Camponotus fellah using Gas chromatography–mass spectrum. International Journal of Toxicological and Pharmacological Research. 8:434-439.

Zhang, L., C. Zhang, Z. Cheng, Y. Yao and J. Chen. 2013. Biodegradation of benzene, toluene, ethylbenzene, and o-xylene by the bacterium Mycobacterium cosmeticum byf-4. Chemosphere. 90:1340-1347.

Published

2019-12-30

How to Cite

Javaid, A., Khan, I. H., Jabeen, K., & Bashir, U. (2019). EVALUATION OF MYCOCHEMICAL PROFILE OF ALTERNARIA JAPONICA THROUGH GC-MS ANALYSIS. Pakistan Journal of Phytopathology, 31(2), 171-175. Retrieved from https://www.pjp.pakps.com/index.php/PJP/article/view/537

Issue

Vol. 31 No. 2 (2019): Pak. J. Phytopath.

Section

Research Articles

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