Sidaoui Abouamama, Bertella Anis, Terbeche Ryme, Haouhach Sadika, El Goumi Younes, Charouine Morad, Moussaoui Rahma, Ben C.M. Elamin


The present work investigated the ability of antagonistic microorganisms to inhibit the growth of pathogenic strains responsible for the vascular fusarium wilt of date palm or Bayoud's disease. The objective was to evaluate the antagonistic effect of native strains of Trichoderma longibarchiatum, Penicillium sp., Fusarium sp. and one isolate of Bacillus sp. against four isolates of Fusarium oxysporum f.sp. albedinis (Foa) under in vitro conditions.

The isolates Bacillus sp. and Fusarium sp. were isolated from soil samples of the rhizosphere of date palm of Tamanrasset region in Algeria, these strains were morphologically identified and a dual culture confrontation was carried out, placing the Foa in the center of the Petri dish and Bacillus sp. in the two cardinal points. For T. longibarchiatum, Penicillium sp. and Fusarium sp. they were confronted against Foa with a dual confrontation, the inhibition rates were determined, and a percentage of inhibition obtained varied from 64.29 to 65.75%; 57.14 to 64.29% and 36.36 to 52.05% for T. longibarchiatum, Penicillium sp and Fusarium sp. respectively. While Bacillus sp. exhibits the lowest percentage of inhibition in this study, and they were less than 16.67%.Comparison of mean value for each pair between T. longibrachiatum, Penicillium sp., Fusarium sp. on one hand and Bacillus sp. on the other hand concluded that there is a highly significant difference between these three fungal isolates and Bacillus sp. concerning inhibition rates with P-value (P <0.0001). Contrary, no significant difference was observed between T. longibrachiatum and Penicillium sp. with P = 0.1895 and the two antagonists show the same letter A in the letter connection ratio, which means no difference in the level of inhibition.


Algerian date palm; Bacillus sp.; Bayoud; Confrontation; Fusarium oxysporum f.sp. albedinis; Fusarium sp.; Penicillium sp.; Trichoderma longibarchiatum

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Bekkar, A.A., L. Belabid and S. Zaim. 2016. Biocontrol of Phytopathogenic Fusarium spp. by Antagonistic Trichoderma. Biopesticide International, 12(1): 37–45.

Belhi, Z., N. Boulenouar, A. Cheriti and A. Marouf. 2020. Antifungal and anti-cellulases activity of Limoniastrum feei extracts to promote Bayoud disease treatment using bioautography. Cogent Food and Agriculture, 6(1): 1–12.

Benabbes, R., I. lahmass, F. Souna, A. Chafi, M. El Youbi, E. Saalaoui, A. Hakkou and M. Bouakka. 2015. Confrontation of antagonistic fungi selected from the soil of the Figuig oasis to date palm Fusarium oxysporum f.sp. albedinis. Moroccan Journal of Biology, 12: 1–8.

Benouzza, S., M. Bellahcene and Z. Fortas. 2020. Biocontrol of Verticillium dahliae by native Trichoderma strains isolated from Algeria. Mycopath, 18(2): 59–70.

Ben Slama, H., H. Cherif-Silini, A.C. Bouket, M. Qader, A. Silini, B. Yahiaoui, F.N. Alenezi, L. Luptakova, M.L. Triki, A. Vallat, T. Oszako, M.E. Rateb and L. Belbahri. 2019. Screening for Fusarium antagonistic bacteria from contrasting niches designated the endophyte Bacillus halotolerans as plant warden against Fusarium. Front. Microbiol, 9: 1–24.

Boughachiche, F., S. Reghioua, L. Oulmi, H. Zerizer, M. Kitouni and A. Boudemagh. 2005. Isolation of actinomycetes producer the antimicrobial substances from sebkha of ain melila. Science and Technology, 23: 5–10.

Bustamante, D.E., M.S. Calderon, S. Leiva, J.E. Mendoza, M. Arce and M. Oliva. 2021. Three new species of Trichoderma in the Harzianum and Longibrachiatum lineages from Peruvian cacao crop soils based on an integrative approach. Mycologia, 1–17.

Brown, D.W. and R.H. Proctor. 2013. Fusarium: Genomics, Molecular and Cellular Biology. Caister Academic Press. Orfolk. UK.

Bahriz, H. and N. Bouras. 2020. Etude de la maladie du bayoud, le comportement variétal du palmier dattier vis-à-vis du Fusarium oxysporum f.sp. albedinis. Africain review of science, technologie and développement, 5 (1): 41– 60.

Chen, J., L. Zhou, I.U. Din, Y. Arafat, Q. Li, J. Wang, T. Wu, L. Wu, H. Wu, X. Qin, G.R. Pokhrel, S. Lin and W. Lin. 2021. Antagonistic activity of Trichoderma spp. against Fusarium oxysporum in rhizosphere of radix pseudostellariae triggers the expression of host defense genes and improves its growth under long-term monoculture system. Frontières in Microbiolog, 12: 1–12.

Denis, F., C. Vincent, M. Christian, P. Marie-Cécile and P. Claire. 2016. Bactériologie médicale: Techniques usuelles. Elsevier /Masson, 2ème Edition, France.

Dukare, A. and S. Paul. 2021. Biological control of Fusarium wilt and growth promotion in pigeon pea (Cajanus cajan) by antagonistic rhizobacteria, displaying multiple modes of pathogen inhibition. Rhizosphere, 17: 1–10.

El Modafar, C. 2010. Mechanisms of date palm resistance to Bayoud disease: Current state of knowledge and research prospects. Physiological and Molecular Plant Pathology, 74: 287–294.

Essarioui, A., H. Ben-Amar, S. Khoulassa, R. Meziani, A. Amamou and F. Mokrini. 2018. Gestion du bayoud du palmier dattier dans les oasis marocaines. Revue Marocaine des Sciences Agronomiques et Vétérinaires, 6 (4): 537–43.

Girish, K. and B.M. Bhavya. 2018. Antifungal activity of Lantana camara L., rhizosphere bacteria. Eurasia Journal of Bioscience, 12: 245–251.

Hammad, M., T. Guillemette, M. Alem, F. Bastide and M. Louanchi. 2021. First report of three species of Trichoderma isolated from the rhizosphere in Algeria and the high antagonistic effect of Trichoderma brevicompactum to control grey mould disease of tomato. Egyptian Journal of Biological Pest Control, 31: 1–11.

Harba, M., M. Jawhar and M.I.E. Arabi. 2020. In vitro antagonistic activity of diverse Bacillus species against Fusarium culmorum and F. solani pathogens. The Open Agriculture Journal, 14(1): 157–63.

Haouhach, S., N. Karkachi, B. Oguiba, A. Sidaoui, I. Chamorro, M. Kihal and E. Mont. 2020. Three New Reports of Trichoderma in Algeria: T. atrobrunneum, (South) T. longibrachiatum (South), and T. afroharzianum (Northwest). Microorganisms, 8: 1–14.

Hibar, K., M. Daami-Remadi, H. Khiareddine and M. El Mahjoub. 2005. Effet inhibiteur in vitro et in vivo du Trichoderma harzianum sur Fusarium oxysporum f.sp. radicislycopersici. Biotechnology, Agronomy, Society and Environment, 9(3): 163–171.

Hussain, M. and N.J. Ismailli. 2020. Isolation and characterization of Fusarium oxysporum f.sp. albedinis causing Bayoud disease of date palm tree from Gambat, District Khairpur, Sindh, Pakistan. Plant Protection, 4 (1): 43–47.

Howell, C.R. 2003. Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts. Plant Disease, 87: 4–10.

Jangir, M., R. Pathak and S. Sharma. 2018. Biocontrol mechanisms of Bacillus sp., isolated from tomato rhizosphere, against Fusarium oxysporum f.sp. Lycopersici. lycopersici, Biological Control, 1–36.

Jeger, M., C. Bragard, D. Caffier, T. Candresse, E. Chatzivassiliou, K. Dehnen-Schmutz, G. Gilioli, J.C. Gregoire, J.A. Jaques Miret, A. MacLeod, N.M. Navajas, B. Niere, S. Parnell, R. Potting, T. Rafoss, G. Urek, A. Van Bruggen, W. Van der Werf, J. West, S. Winter, J. Armengol Forti, I. Vloutoglou, B. Bottex and V. Rossi. 2018. Scientific Opinion on the pest categorisation of Fusarium oxysporum f.sp. albedinis. EFSA Journal, 16: 1–24.

Kumar, A., S.P.J. Kumar, D.V. Chintagunta, D.K. Agarwal, G. Pal, A.N. Singh and J.S. Gandara. 2021. Biocontrol potential of Pseudomonas stutzeri endophyte from Withania somnifera (Ashwagandha) seed extract against pathogenic Fusarium oxysporum and Rhizoctonia solani. Archives of Phytopathology and Plant Protection, 1–18.

Lahlali, R., W. Aksissou, N. Lyousfi, S. Ezrari, A. Blenzar, A. Tahiri, S. Ennahli, J. Hrustić, D. Maclean and S. Amiri. 2020. Biocontrol activity and putative mechanism of Bacillus amyloliquefaciens (SF14 and SP10), Alcaligenes Faecalis ACBC1, and Pantoea Agglomerans ACBP1 against Brown Rot Disease of Fruit. Microbial Pathogenesis, 139: 1–53.

Leslie, J.F. and B.A. Summerell. 2006. The Fusarium Laboratory Manual. Copyright © 2006 Blackwell Publishing, 1ed.

Liu, Y.T., I.C. Yang and N.C. Lin. 2020. Evaluation of biocontrol potential for Fusarium yellows of celery by antagonistic and gallic acid-degrading bacteria. Biological Control, 146: 1–9.

Lounaci, L. and S. Athmani-Guemouri. 2014. Action de Paenibacillus polymyxa SGK2 sur quelques champignons de la fusariose du blé dur (Triticum durum) en Algérie. Algerian Journal of Natural Products, 2(2): 35–42.

Marimuthu, S., C. Karthic, A.M. Ashraf, N.M. Al-Enazi, A. Neveen and E.N. Sholkamy. 2020. Antifungal activity of Streptomyces sp. SLR03 against tea fungal plant pathogen Pestalotiopsis theae. Journal of King Saud University – Science, 32: 3258–3264.

Metlo, W.A., G.H. Jatoi, G.S. Channa, N.W. Awan, M.A. Abro, B.M.A. Hidayatullah, M.H. Memon, M.I. Chandio and A. Siddiqua. 2019. Pathogenicity test of different fungi on date palm causing sudden decline disease of date palm (Phoenix dactylifera L.) in Khairpur, Pakistan. International Journal of Biosciences, 15(6): 89–96.

Metlo, W.A., G.S. Markhand, Z.A. Chandio, Q.U.A. Shaikh, L. Bux and W.A. Jatoi. 2021. Occurrence of sudden decline disease of date palm (Phoenix dactylifera L.) in khairpur, Pakistan. Pakistan Journal of Phytopathology, 33(01): 75–81.

Mohiddin, F.A., S.A. Padder, A.H. Bhat, M.A. Ahanger, A.B. Shikari, S.H. Wani, F.A. Bhat, N. Sajad, A. Hamid, N.A. Bhat. 2021. Phylogeny and Optimization of Trichoderma harzianum for Chitinase Production: Evaluation of Their Antifungal Behaviour against the Prominent Soil Borne Phyto-Pathogens of Temperate India. Microorganisms, 9: 1–20

Njenga, W.P., F.B. Mwaura, J.M. Wagacha and E.M. Gathuru. 2017. Methods of isolating Actinomycetes from the soils of Menengai Crater in Kenya. Arch Clin Microbiol, 8(3): 1-7.

Nicoletti, R. and M. De Stefano. 2012. Penicillium restrictum as an Antagonist of Plant Pathogenic Fungi. Dynamic Biochemistry, Process Biotechnology and Molecular Biology, 6(2): 61–69.

Oubella, K., B. Ouhmidou, M. Ait Chitt and M. Moumni. 2017. Isolation and morphological identification of desert arbuscular mycorhizal fungi from Errachidia region in Morocco. International Journal of Pharmacy and Biological Sciences, 8(3): 105–113.

Oubraim, S., M.H. Sedra and H.B. Lazrek. 2016. A relationship between Bayoud disease severity and toxin susceptibility of date palm cultivars. Emirates Journal of Food and Agriculture, 28(1): 45–51.

Pecundo, M.H., T.E.E. dela Cruz, T. Chen, K.I. Notarte, H. Ren and N. Li. 2021. Diversity, phylogeny and antagonistic activity of fungal endophytes associated with endemic species of Cycas (cycadales) in China. Journal of Fungi, 7: 1–25.

Pani, S., A. Kumar and A. Sharma. 2021. Trichoderma harzianum: An Overview. Bulletin of Environment, Pharmacology and Life Science, 10(6): 32–39.

Putri, R.E., N.R. Mubarik, L. Ambarsari and A.T. Wahyudi. 2021. Antagonistic activity of glucanolytic bacteria Bacillus subtilis W3.15 against Fusarium oxysporum and its enzyme characterization. Biodiversitas, 22: 4067–4077.

Rajathi, S., S. Murugesan, V. Ambikapathy and A. Panneerselvam. 2020. Antagonistic activity of fungal cultures filtrate and their enzyme activity against Fusarium moniliforme causing bakanae diseases in paddy. International Journal of Botany Studies, 5: 654–658.

Sghir, F., J. Touati, B. Mouria, A.O. Touhami, A. Filali-Maltouf, C. El Modafar, A. Moukhli, R. Benkirane and A. Douira. 2016. Variation in pathogenicity of Fusarium oxysporum f.sp. albedinis on two cultures associated with date palm of Moroccan oasis. World Journal of Pharmaceutical and Life Sciences, 2(3): 56–68.

Sidaoui, A., N. Karkachi, A. Bertella, S. Boudeffeur, M. Chhiba, A. Tebyaoui, Y. El Goumi and M. Kihal. 2017. Morphological study and caracterisation of Fusarium oxysporum f.sp. albedinis by isozymes systems. International Journal of Scientific & Engineering Research, 8: 1764–1768.

Sidaoui, A., N. Karkachi, A. Bertella, Y. El Goumi, S. Haouhach, B. Oguiba, S. Boudeffeur, M. Chhiba and M. Kihal. 2018. Pathogenicity and biological control of bayoud disease by Trichoderma longibrachiatum and Artemisia herba-alba essential oil. Journal of Applied Pharmaceutical Science, 8(04): 161-67.

Tian, Y., D. Yu, N. Liu, Y. Tang, Z. Yan and A. Wu. 2020. Confrontation assays and mycotoxin treatment reveal antagonistic activities of Trichoderma and the fate of Fusarium mycotoxins in microbial interaction. Environmental Pollution, 267: 1–11.

Tiru, Z., M. Sarkar, A.P. Chakraborty et al. 2021. In vitro antagonistic study of maize root colonizing fungal isolates against Fusarium moniliforme causing ear rot disease of Maize. Journal of Tropical Life Science, 11 (2): 133–139.



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